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Artificial hybridization and breeding method for male Paralichthys dentatus and female summer flounder

A technology of artificial hybridization and flounder, which is applied in the field of fish breeding and aquaculture, can solve the problems of unseen flounder and flounder, etc., and achieve the effects of outstanding excellent characters, large variation range, and normal growth and development.

Inactive Publication Date: 2006-10-11
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

At present, there are no related reports on the hybridization of summer flounder and flounder

Method used

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Experimental program
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Embodiment

[0030] 1. Materials and methods

[0031] (1) Male parent cultivation: male adult fish of summer flounder above the second age was selected as the male parent. In November 2004, gonad maturation intensive cultivation began. The broodstock breeding pond is a 5.5×5.5×1.2m cement pond. The natural seawater is extracted, and after sedimentation, sand filtration, and heating, the daily water change is 2 full volumes. Feed ice-fresh jade-tenon fish and squid, add broodstock nutritional enhancer (combo A produced by Qingdao Senmiao Industrial Co., Ltd.) accounting for 3% of the bait weight, and feed twice a day (8:00 and 15:00). The surface light of the broodstock pond is completely controlled by light, the light intensity is 60-500Lux, the light time is transitioned from 12L / 12D to 8L / 16D short light, and the water temperature is 17-19°C.

[0032] (2) Breeding of the female parent: select adult female flounder over the age of three as the female parent. In December 2004, prenatal...

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Abstract

The invention belongs to field of fish breeding and aquaculture, which in detail relates to a method of artificial crossing with summer-bothus and tooth-Bothus and breeding. It comprises following steps: (1) taking summer-bothus as male parent and tooth-bothus as female parent, reinforcing the nutrient and environmental condition to promote simultaneous gonadal maturity, getting mature sperm and ovum; (2) crossing fertilized ovum and hatching; (3) culturing the hybrid body and young fish; (4) providing systematic bait in culturing period. The invention not only provides material for deseription improvement for bothus and soles, but also the lies foundation of adding a new breeding species for sea fish cultivating.

Description

technical field [0001] The invention belongs to the field of fish breeding and aquaculture. Specifically speaking, it is an artificial hybridization method using the sperm of summer flounder and flounder eggs, the hatching of hybrid fertilized eggs, and the artificial cultivation of hybrid larvae and juvenile fish. Background technique [0002] Hybridization can drastically shake the conservativeness of heredity, making the heredity of hybrids more mobile, more plastic, and have the possibility of developing into all aspects of human cultivation. Hybridization not only rapidly and significantly improves the viability of hybrids and obtains heterosis, but also enriches the genetic structure by freely combining the genes of more than two varieties or individuals of more than one species with different genetic bases to form new genetic types, and then people can select good ones. Individuals are bred to form new varieties. Therefore, hybrid breeding can add more excellent new...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 肖志忠李军马道远于道德徐世宏
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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