Microcapsule preparing process utilizing waste beer yeast cell as wall material
A technology for beer waste yeast and yeast cells, which is applied in the field of microcapsule preparation, can solve the problems of high cost of microcapsules, difficult drying, difficult granulation operation, poor dispersibility, etc., and achieves reduced preparation cost, low cost of raw materials, and high entrapment rate. Effect
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Embodiment 1
[0012] Example 1 Wash the waste beer yeast recovered by the fermentation factory with 3 times of sterile water, pass through 80 mesh (diameter) sieves, remove impurities with larger particle diameters, and collect the thalline with 3000r / min centrifugation for 10min; follow the same steps Rinse the bacteria with sterile water for 2 times; collect the yeast precipitate, add water to make a 10% suspension; use a centrifugal atomizer with a rotation speed of 9500r / min, send the yeast suspension into a dryer for drying, and obtain Dry yeast granules with a diameter of about 0.5-2 mm and a moisture content of less than 2%.
[0013] Precisely weigh 2g of dry yeast, put it into a ground-mouthed Erlenmeyer flask, add 2.0g of cinnamaldehyde liquid, cover it and put it into a constant temperature water bath oscillator; shake it at 50°C (speed 80r / min) for 4h, and it can pass through the osmotic diffusion It enters the inside of yeast cells to form microcapsules; suction filters and reco...
Embodiment 2
[0015] Example 2 The purification and drying process of waste beer yeast is the same as in Example 1. Accurately weigh 2g of dry yeast, put it into a ground-mouthed Erlenmeyer flask, add 2g of clove oil, put it into a constant temperature water bath shaker after being capped; at 70°C (speed 80r / min) shake for 9h; suction filter and recover the unencapsulated clove oil; wash the essential oil remaining on the cell surface with absolute ethanol; vacuum dry at 30°C for 20min; collect the microcapsules and weigh them. The embedding rate of clove oil can reach 41.26%. Example 3 The purification and drying process of spent beer yeast is the same as in Example 1. Precisely weigh 2.0g of dry yeast, put it into a grinding-mouth Erlenmeyer flask, add 2.5g of rosemary essential oil, cover it and put it into a constant temperature water bath shaker; shake at 60°C (speed 100r / min) for 6h to form Microcapsules; suction filtration and recovery of unencapsulated essential oils; absolute etha...
Embodiment 4
[0016] Example 4 The purification and drying process of spent beer yeast is the same as in Example 1. Accurately weigh 2.0g of dry yeast, put it into a grinding mouth Erlenmeyer flask, add 2ml of 1mol / L ethanol solution of phenyl salicylate, cover it and put it in a constant temperature water bath shaker; Shake for 6 hours to form microcapsules; wash the solution remaining on the cell surface with absolute ethanol; vacuum dry at 40°C for 60 minutes; collect the microcapsules and weigh them. The embedding rate of phenyl salicylate can reach 67.64%.
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