Detection of transcription factor protein by double chain RNA molecule with special labeling fixed to micro-plate coated by exonuclease III

Abstract

Transcription factor is one important protein for gene regulation, is center of gene expression regulating passage and network, is important target of functional genome and protein block research and important target site of transcription treatment and medicine research. The present invention proposes exonuclease III digesting double stranded nucleic acid molecule coated in microporous plate and containing special marker to detect transcription factor protein. The process of analyzing transcription factor expression and activation level includes the following steps: preparing nucleic acid; fixing the nucleic acid molecules to the pores of the microporous plate; incubation of cell or tissue extract containing transcription factor in the plate; incubation of exonuclease III reaction liquid in the plate; incubation of specific conjugate with special marker on nucleic acid on the plate; detecting and analyzing the specific conjugate to obtain the expression and activation level.

Description

1. Technical field: [0001] Transcription factor (transcription factor) is a kind of important protein responsible for gene expression regulation in proteomics. ) and proteome research; many diseases are closely related to the abnormal expression and activation of transcription factors, and have become important targets for transcriptional therapy and drug research (drug target). The detection and analysis of transcription factor expression and activation degree is the main means to study its function. This patent proposes a new method for detecting the expression and activation level of transcription factors, which will be used in the research of transcription factors in the fields of molecular biology, functional genomics, proteomics and biomedicine Provide a new detection and analysis technology that can promote scientific research on transcription factors in these fields, and provide a diagnostic technology for disease-related transcription factors in the biomedical field,...

AI Technical Summary

Problems solved by technology

Although our double-stranded DNA microarray chip technology has reached the practical level, we noticed that the popularization and application of this technology still faces great difficulties, that is, the cost of chip preparation and use is relatively high

In particular, the actual use of the chip must rely on the current very expensive gene chip scanning device, which greatly limits the commercialization of double-stranded DNA microarray chip technology

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