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Secreted acid phosphatase (SAPM) is present only in pathogenic mycobacteria and expressed selectively at phagosomal PH

A technology of acid phosphatase and mycobacteria, which is applied in the direction of bacterial antigen components, medical preparations containing active ingredients, enzymes, etc., and can solve the problems of unknown biological functions of genes and unclear direct effects of bacteria

Inactive Publication Date: 2010-07-21
成都赛普克生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although many candidate pathogenic genes have been described, the biological functions of most of them are still unknown, and their direct effect on the survival of bacteria in cells is still unclear

Method used

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  • Secreted acid phosphatase (SAPM) is present only in pathogenic mycobacteria and expressed selectively at phagosomal PH
  • Secreted acid phosphatase (SAPM) is present only in pathogenic mycobacteria and expressed selectively at phagosomal PH
  • Secreted acid phosphatase (SAPM) is present only in pathogenic mycobacteria and expressed selectively at phagosomal PH

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Embodiment Construction

[0041] The present invention will now be described more fully with reference to the accompanying drawings, and a preferred embodiment thereof will be shown. However, the invention can also be implemented in different embodiments and should not be construed as being limited to the embodiments set forth in this application. Rather, these embodiments are provided so that this specification will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

[0042] The term "antibody" used in this application includes not only the antibody itself, but also biologically active derivatives of the antibody, such as Fab', F(ab').sub.2 or Fv, as well as single-domain and single-chain antibodies. Biologically active derivatives of antibodies retain the ability to bind antigen.

[0043] The term "isolated DNA sequence" as used herein includes DNA single or double strands. Said sequence is isolated and / or purified (i.e. from a natural environmen...

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Abstract

The invention relates to an isolated DNA construct, comprising a mycobacterium secreted acid phosphatase promoter or promoter fragment which is inducible by low pH. This invention further relates to diagnostic methods and vaccines for treatment or prophylaxis of pathogenic mycobacterial disease or infection. This invention also provides for methods of screening for compounds capable of modulatingthe activity, production or secretion of a mycobacterium secreted acid phosphatase.

Description

technical field [0001] The present invention relates to diagnostic methods and vaccines for pathogenic mycobacterial or pathogenic fungal diseases or infections. Background technique [0002] Pathogenic mycobacteria, including Mycobacterium tuberculosis, colonize the phagosome of host macrophages, a hostile environment for most microorganisms. Apparently Mycobacterium tuberculosis has altered this phagosomal compartment to enhance its own intracellular survival (Clemens and Horwitz, 1995). These include changes in the composition of Rab GTPase (Via et al., 1997; Clemens et al., 2000) and the excretion of vacuolar proton ATPase (Sturgill et al., 1994). Thus, mycobacterial phagosomes are captured early in maturation and are only slightly acidified to pH 6.1-6.5 (Xu et al., 1994; Sturgillet et al., 1994; Clemens and Horwitz, 1995). The persistence of pathogenic mycobacteria in macrophages plays a key role in disease initiation and progression. But the specific bacterial fact...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/16C12N15/55C12N15/67C12N5/10C07K16/12G01N33/50A61K39/04A61K38/00A61K39/00C12N15/52C12N15/57C12N15/63
CPCA61K38/00C12Y301/03002C12N9/16C12N15/52A61K39/00A61P31/04A61P31/06A61P43/00A61K39/04A61K39/40C07K16/1289G01N33/5695
Inventor 刘军马赞·T·萨利赫大卫·亚历山大
Owner 成都赛普克生物科技股份有限公司
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