Nucleic acid adaptor for specific identifying human cerebral acetylcholinesterase and its use

Abstract

The invention offers oligonucleotide adapter of specific combining human-brain acetylcholinesterase containing SEQ ID: 1, SEQ ID: 2 or SEQ ID: 3 sequence, method of detecting or discriminating human-brain acetylcholinesterase, method and diagnostic reagent and medicine to diagnose or therapy the disease relating to human-brain acetylcholinesterase (especially neural tube defect and neurotic disorder), and so on.

Description

technical field The present invention mainly relates to oligonucleotide aptamers specifically binding to human brain acetylcholinesterase, compositions containing it, methods for detecting or identifying human brain acetylcholinesterase, and diagnosis or treatment of diseases related to human brain acetylcholinesterase ( In particular, methods and diagnostic reagents and drugs for neural tube defects and neurological disorders). Background technique SELEX technology is a new combinatorial chemical technology developed in recent years to study the structure, function and evolution of nucleic acids. It not only has good applications in basic research and biological screening, but also shows broad potential in clinical diagnosis. Prospects (Brody EN & Gold L, J. Biotechnol., 2000, 75:5-13). The screened oligonucleotide aptamers (aptamers) can recognize different molecules with high affinity and specificity. Its affinity and specificity are comparable to those of antibodies, a...

AI Technical Summary

Problems solved by technology

Amniocentesis may cause false positives due to blood sample contamination, so reagents that can distinguish AChE from human brain and AChE from human erythrocytes are very useful in prenatal diagnosis of NTD; (3) B-ultrasound and other methods are not applicable in early pregnancy

Images