Barm extract for enhancing soup stock flavor
A yeast extract and yeast technology, applied in food science, food preparation, application, etc., can solve problems such as imbalance, failure to highlight the thick and mixed flavor of broth, and unfavorable broth taste.
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Embodiment 1
[0024] With a 300-liter fermenter, baker's yeast cells (Saccharomyces cerevisiae (Saccharomyces cerevisiae) 3-2-C-7, FERM BP-10022, preserved in the National Institute of Advanced Industrial Science and Technology and Technology), add water in yeast cell, prepare the suspension containing 10% solid matter, regulate the pH value of cell suspension to 5.7 with 40% NaOH solution.Use cell wall digestion enzyme YL-15 (Amano enzyme preparation company) with solid content Add 0.1% and react at 45°C for 8 hours and 10 hours respectively. After the reaction is completed, the reaction product is heated at 70°C for 30 minutes to inactivate the enzyme, and then cooled to 40°C. After that, the peptidase R (Amano Enzyme Company) was added with a solid content of 0.2%, and reacted for 2 hours at 40°C. After the reaction was completed, the reaction product was heated at 70°C for 30 minutes to inactivate the enzyme, and the creamy yeast extract was obtained with traditional techniques.
[0025...
Embodiment 2
[0034]Baker's yeast cells (Saccharomyces cerevisiae HG-1, a variant of 3-2-C-7 cells that produce large amounts of glutamate) were grown by conventional techniques in a 300-liter fermenter. Add water to the yeast cells to prepare a suspension containing 10% solids, and adjust the pH value of the cell suspension to 5.7 with 40% NaOH solution. Cell wall digestion enzyme YL-15 (Amano Enzyme Co., Ltd.) was added at a solid content of 0.1%, and reacted at 45° C. for 8 hours. After the reaction was completed, the reaction product was heated at 70°C for 30 minutes to inactivate the enzyme, and then cooled to 40°C. Thereafter, peptidase R (Amano Enzyme Co., Ltd.) was added at a solid content of 0.2%, and reacted at 40° C. for 2 hours. After completion of the reaction, the reaction product was heated at 70° C. for 30 minutes to inactivate the enzyme, and then the product was heated at 70° C. for another 30 minutes, thereby obtaining a creamy yeast extract by conventional techniques. ...
Embodiment 3
[0069] Using a 300-liter fermenter, culture Baker's yeast cells (Saccharomyces cerevisiae 3-2-C-7) by traditional techniques, add water to the yeast cells to prepare a suspension containing 10% solids, and adjust the cell suspension with 40% NaOH solution solution pH to 5.7. Cell wall digestion enzyme YL-15 (Amano Enzyme Co., Ltd.) was added at a solid content of 0.1%, and reacted at 45° C. for 8 hours. After the reaction was completed, the reaction product was heated at 70°C for 30 minutes to inactivate the enzyme, and then cooled to 45°C. Thereafter, Neutrase F3G (Amano Enzyme Co., Ltd.), which has weaker exoproteinase activity, was added at a solid content of 0.2%, and reacted at 45° C. for 1 hour and 2 hours, respectively. After the reaction was completed, the reaction product was heated at 70° C. for 30 minutes to inactivate the enzyme, and a creamy yeast extract was obtained by conventional techniques.
[0070] The composition ratio of the obtained yeast extract determ...
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