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Tall fescue regeneration plant obtaining method through tissue culture

A technology for regeneration of plants and tissue culture, applied in the biological field, can solve the problems of significant differences in regeneration ability and difficult regeneration of varieties

Inactive Publication Date: 2005-04-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the deficiencies and defects of the background technology, overcomes the problems of difficult regeneration in the tissue culture process of tall fescue, and the significant difference in regeneration ability between varieties, etc., and designs suitable It is a medium for the regeneration of most varieties of tall fescue, and through a certain experimental process, the callus state and development direction of tall fescue can be controlled, and the differentiation rate of tall fescue callus can be greatly improved (up to 96%) and The acquisition rate of regenerated plants (more than 30 regenerated shoots can be obtained from each piece of callus on the differentiation medium), and the time to complete the regeneration of tall fescue plants is only about 10 weeks

Method used

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  • Tall fescue regeneration plant obtaining method through tissue culture
  • Tall fescue regeneration plant obtaining method through tissue culture
  • Tall fescue regeneration plant obtaining method through tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The American tall fescue cultivar 'surpro' was used as the test material, which was purchased from the Lawn Research Institute of China Agricultural University. Choose 150 plump seeds, soak them in 75% alcohol for 3 minutes after removing their shells, then disinfect them with 0.1% mercuric chloride for 10 minutes, and then wash them thoroughly with sterile distilled water 5 times. Disinfected seeds were inoculated into the callus induction medium MSM containing 5mg / L, 7mg / L, 9mg / L 2,4-D and three treatments of 0.025mg / L KT (50 seeds for each treatment). Wounded tissue induction culture. After 2 weeks, the calli induced by each treatment were transferred to the callus maintenance medium containing 4.5mg / L2, 4-D and 0.2mg / L KT in MSM for proliferation. After 3 weeks of callus proliferation and culture, they were divided into mung bean sizes and transferred to MSM medium containing 2mg / L 2,4-D and 0.2mg / L KT (hydrolyzed casein content was 1mg / L) for somatic embryogenesis...

Embodiment 2

[0028]The American tall fescue cultivar 'Coronado' was purchased from the Lawn Research Institute of China Agricultural University. Choose 150 plump seeds, soak them in 75% alcohol for 3 minutes after removing their shells, and then disinfect them with 0.1% mercuric chloride for 10 minutes, and then wash them thoroughly with sterile distilled water 5 times. The sterilized seeds were inoculated into the callus induction medium MSM containing 6mg / L, 8mg / L, 10mg / L 2, 4-D and 0.025mg / L KT under three treatments (50 seeds for each treatment). Wounded tissue induction culture. After 2 weeks, the callus formed under each treatment was transferred to the callus maintenance medium containing 4.5mg / L2, 4-D and 0.2mg / L KT (hydrolyzed casein content was 2mg / L) Proliferate. After callus proliferation and culture for 4 weeks, the callus with somatic embryos was inoculated on the differentiation medium MSH+2mg / L KT+2% sucrose (the content of hydrolyzed casein was 2mg / L) to differentiate in...

Embodiment 3

[0030] The European cultivar 'Summer Lawn' was used as the test material, which was purchased from the Lawn Research Institute of China Agricultural University. Choose 150 plump seeds, soak them in 75% alcohol for 3 minutes after removing their shells, then disinfect them with 0.1% mercuric chloride for 10 minutes, and then wash them thoroughly with sterile distilled water 5 times. Disinfected seeds were inoculated into the callus induction medium MSM containing 5mg / L, 7mg / L, 9mg / L 2,4-D and three treatments of 0.025mg / L KT (50 seeds for each treatment). Wounded tissue induction culture. After 2 weeks, the callus formed under each treatment was transferred to the callus maintenance medium containing 4.5mg / L2, 4-D and 0.2mg / L KT (hydrolyzed casein content was 1mg / L) Proliferate. After 3 weeks of callus proliferation and culture, they were transferred to MSM medium containing 2mg / L 2,4-D and 0.1mg / L KT (hydrolyzed casein content was 1mg / L) for induction culture of somatic embr...

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Abstract

This invention presents a way to get a lot of GaoYangMao regrow plant through organizing and cultivate. This method is choosing ripped GaoYangMao seeds antisepticising the seeds then cultivate on the advanced cultivation basis, and let it grow to embryo to advanced polarization cultivate basis, let the cell an embryo tissue grow to a whole plant, then plant it into pot, accomplish the whole process. This way is fit for most GaoYangMao regrow. We get the regrow plant frequently with short period in growing, is fit for advanced inheritance.

Description

technical field [0001] The invention relates to a method for obtaining a large amount of tall fescue regenerated seedlings by using tissue culture technology, and belongs to the field of biotechnology. Background technique [0002] Tall fescue is an important high-nutrient cool-season forage grass and an important turfgrass in the world. It has the characteristics of drought resistance, barren resistance, disease resistance and wide adaptability, and is widely used in urban greening and sports lawn establishment. But it also has the disadvantages of rough leaves, no stolons, poor lawn expansion, and slow growth in summer. Biotechnology can be used to efficiently and quickly genetically improve the traits of crop varieties, thus providing a convenient, fast and effective method for tall fescue breeding. Because the key to biotechnology breeding is to establish an efficient tissue culture system to provide a gene receptor system for improving target traits and a culture syst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王涛张万军
Owner CHINA AGRI UNIV
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