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Method for culturing potato virus-free test-tube plantlets in water culture mode

A potato and test-tube seedling technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow plant growth, variety variation, and high substrate cost, and achieve the effect of improving production efficiency

Active Publication Date: 2012-09-12
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]2. A method for rapid propagation of cuttings and strong seedlings in potato matrix cultivation, the main steps of which are: (1) hardening potato seedlings in test tubes free of viruses; (2) Clean and root the virus-free test-tube seedlings, soak them in 100mg / L NAA solution for 15 minutes after cleaning; ℃, humidity above 95%; (4) When the greenhouse temperature is 0-10℃, it takes 40-50 days for the seedlings to grow to about 12cm, and when the temperature is higher than 30℃, the survival rate is lower than 50%.
[0007]The disadvantages of the above-mentioned technologies are poor controllability and high risk
After rapid propagation in the tissue culture room, it is necessary to add hormones to strengthen the seedlings, which will cause the risk of variety variation
In addition, the hormones added to strong seedlings in tissue culture are all growth retardants, and the plants grow slowly. Even if the effect of strong seedlings can be achieved, it will take a long time. The robustness of seedlings is far inferior to that of hydroponic seedlings
For the substrate cultivation of virus-free seedlings, the cost of the substrate is high, and it is difficult to thoroughly disinfect after repeated use, and the possibility of infection by diseases and insect pests is very high
Generally, the survival rate of matrix cultivation is lower than that of hydroponics, and matrix cultivation needs to be carried out in large insect-proof greenhouses, which often cannot be well controlled by humans for temperature, humidity and light intensity. When the temperature is low, the growth of matrix seedlings is extremely slow. Virus-free seedlings that are too high are not easy to survive, so substrate cultivation cannot perform rapid propagation and strong seedlings well.

Method used

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  • Method for culturing potato virus-free test-tube plantlets in water culture mode
  • Method for culturing potato virus-free test-tube plantlets in water culture mode
  • Method for culturing potato virus-free test-tube plantlets in water culture mode

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specific Embodiment approach

[0026] The specific implementation method comprises the following steps:

[0027] (1) The tissue culture seedlings are cultivated in the tissue culture room for about 25 days, when they are 5-7 cm long, they are then placed at a temperature of 20-22°C and a light intensity of 2000-3000lx for 3-5 days, and the seedlings are hardened and let off. Poison test-tube seedlings adapt to hydroponic growth conditions and improve survival rate;

[0028] Use fluorescent lamps and air conditioners to adjust the temperature and light of the greenhouse, or adjust the temperature with water curtains and steam when the light is sufficient.

[0029] (2) Take out the virus-free seedlings after hardening from the tissue culture bottle, cut off the original root system, wash the remaining medium with clean water, and then soak the roots in a pH value of 5-6 and a concentration of 90-110mg 15-20min in the naphthalene acetic acid solution of / L, carry out rooting treatment;

[0030] (3) Preparati...

Embodiment 1

[0039] Potato virus-free seedling variety is the embodiment of Fei Wu Rui it, has 8 processings, and processing 8 is control group:

[0040] Table 1

[0041]

[0042] The following results are obtained, as shown in the table below:

[0043] Table 2 Differences of various morphological indicators under different treatments of Fevorita

[0044]

[0045] It can be seen from Table 2 that the survival rate, plant height and stem diameter of treatments 1, 2, 3, 4, 5, 6 and 7 were significantly higher than those of control group 8. Wherein the highest survival rate is treatment 6, which is 95%, which is significantly higher than 26% of control treatment 8; the highest plant height is 13.69cm for treatment 5, which is very significantly higher than 7.65cm of control treatment 8; the largest leaf area 4.03 cm for processing 5 2 , which was also significantly larger than the control; the thickest stem was treatment 1, with a diameter of 1.77mm, which was significantly larger t...

Embodiment 2

[0049] Potato virus-free seedling kind is the embodiment of Mira, has 8 treatments, and 8 treatments are control groups:

[0050] table 3

[0051]

[0052] The result is as follows:

[0053] Table 4 Differences of various morphological indicators under different treatments of Mira

[0054]

[0055] As can be seen from Table 4, Mira has strong adaptability, and the survival rate, plant height and stem diameter of treatments 1, 2, 3, 4, 5, 6 and 7 are all higher than those of the control group 8, and there are differences among the treatments. Not significant, only treatment 6 was significantly lower than other treatments in the maximum leaf area.

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Abstract

The invention belongs to the field of potato stock seed culture and particularly relates to a method for culturing potato virus-free test-tube plantlets in a water culture mode. The method comprises the steps of seedling exercising, rooting treatment, nutrition liquid preparation, field planting, culture and transplanting. In aspects of variety, culture density, nutrition liquid concentration and rooting agent concentration, the propagating factor and the strong seedling quantity are increased, corresponding measures are adopted for handling in each link, the propagating factor of virus-free seedlings is improved, healthy and strong virus-free seedlings are obtained, and the soilless culture mode requirements of fog culture, substrates and the like are met, so the healthy and efficient potato stock seed production is reached. The original virus-free, germless and insect-free characteristics of the plantlets are maintained, the characteristics of simple technology, low cost, strong germchits and the like are also realized, in addition, the survival rate of the water culture potato seedlings transplanted to the substrates or other soilless culture (such as fog culture) is very high and can sometimes reach 99 percent, and the production efficiency of potato stock seeds is improved.

Description

technical field [0001] The invention belongs to the field of potato original seed cultivation, and in particular relates to a method for cultivating virus-free test-tube seedlings of potato in a hydroponic manner. Background technique [0002] The original potato seed has the advantages of high variety purity, no virus, good storage and transportation performance, and obvious yield increase effect. Therefore, the production of virus-free potato has become the main measure for rapid seed potato propagation. The production of the original potato seed can be cut by cutting the tip of the virus-free seedlings to increase the reproduction coefficient, so as to speed up the rapid propagation process and reduce the production cost. [0003] At present, there are two main technologies for rapid propagation and strong seedlings of potato virus-free test-tube seedlings. The main status of these two technologies is introduced as follows: [0004] 1. The method of rapid propagation and...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 何卫王克秀胡建军谢开云王余明唐铭霞陈萍萍卢学兰刘冬
Owner CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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