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Application of infrared photosensitizer in the photodynamics therapy

A kind of photosensitizer and tricarbocyanine technology, which is used in the application field of photosensitizer in biomedicine

Inactive Publication Date: 2005-01-26
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there have been no reports of using such infrared dyes for photodynamic therapy.

Method used

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  • Application of infrared photosensitizer in the photodynamics therapy
  • Application of infrared photosensitizer in the photodynamics therapy
  • Application of infrared photosensitizer in the photodynamics therapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] (1) The effect of IR-140 on human oral epithelial squamous carcinoma cells referred to as KB cells (KB cells were purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences)

[0023] 1.MTT experiment

[0024] (1) Cells were seeded in a 96-well plate and cultured (37°C, 5% CO 2 , RPMI1640 medium containing 10% calf serum) overnight to make it adhere to the wall.

[0025] (2) Adding IR-140 at different concentrations of 1-30 μmol / l and incubating for 0.5-2 hours to fully contact the cells with IR-140.

[0026] (3) Halogen lamp at 0.65J / cm 2 Cells were irradiated with high power for 20 minutes.

[0027] (4) Continue to culture the cells for 24 hours (the same conditions as above).

[0028] (5) Remove the culture medium, add 20 μl of 5 mg / ml MTT (purchased from Sigma), and incubate for 4 hours.

[0029] (6) Add cell lysate (50%DMF-20%SDS), after all dissolving, detect absorbance at 570nm place with microplate reader (Bio-Rad company) (results such a...

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Abstract

The invention relates to the use of photosensitizer in biological and pharmacy, wherein tricarbocyanine photosensitizers are utilized into the cell models for treating solid cancer through photodynamics, and in developing medicament for treating tumor.

Description

1: Technical field [0001] The invention belongs to the technical field of the application of photosensitizers in biomedicine. Two: background technology [0002] As infrared dyes, tricarbocyanine compounds have been used as sensitizers for infrared films. They have the characteristics of long absorption wavelength and high sensitivity. In recent years, some tricarbocyanine compounds have been developed as molecular probes and have been successfully used as detection reagents. But so far, there has been no report on using such infrared dyes for photodynamic therapy. Three: Invention content [0003] The problem to be solved in the present invention is to use the tricarbocyanine photosensitizer in the cell model of photodynamic therapy for solid tumors, and to develop, produce and prepare drugs for treating tumors. This type of photosensitizer is a tricarbocyanine substance with the characteristics of long absorption wavelength, high sensitivity, and low biological toxicit...

Claims

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Application Information

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IPC IPC(8): A61K31/41A61K31/423A61K31/428A61P35/00
Inventor 沈萍萍宋远礼陈小平胡一桥徐广智
Owner NANJING UNIV
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