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Process for preparing active peptide using fish skin collagen step directional enzynolysed by composit enzyme

A technology of directional enzymolysis of fish skin collagen, which is applied in the field of preparation of antihypertensive active peptides, can solve the problems of poor enzymatic hydrolysis effect, low active peptide activity, and high raw material price, and achieve reasonable molecular weight distribution, high activity, and low price Effect

Inactive Publication Date: 2005-01-05
OCEAN UNIV OF CHINA
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AI Technical Summary

Problems solved by technology

Many people in China are developing active peptides with blood pressure-lowering function using different raw materials as objects. The main problems at present are the high price of the raw materials used, and the poor enzymatic hydrolysis effect, low activity of active peptides, and low yield

Method used

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Examples

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Embodiment Construction

[0006] Dissolve fish skin collagen in a water bath with 0.02M, pH 4.5 phosphate buffer to obtain a solution with a concentration of 0.05kg / l. After preheating the solution to 45°C, add pineapple at a substrate ratio of 4000U / g Protease, stir, enzymolysis at 45°C for 4 hours; inactivate enzyme at 100°C for 5 minutes, cool to 55°C, adjust pH to 7.5 with NaOH, add Alcalase 2.4L protease at a ratio of 6000U / g substrate, stir, 55 Enzymolysis at a constant temperature of ℃ for 2 hours, inactivation of enzyme at 100℃ for 5 minutes, cooling to room temperature, and lyophilization to obtain a protein hydrolyzate with hypotensive activity, which is separated and purified to obtain a hypotensive active peptide.

[0007] The invention uses alkaline protease to enzymatically hydrolyze fish skin collagen (including fish skin collagen such as crucian carp, bighead carp, tilapia and perch). Since the hydrolysis effect of a single enzyme system is often not good, a compound enzymatic hydrolysi...

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Abstract

The invention is a method of preparing active peptides by making step oriented enzymolysis of compound enzyme on fish skin collagen, and its characteristic: putting the fish skin collagen in a phosphatic buffer solution to prepare a solution at 0.04-0.06kg / l, adding in pineapple proteinase at 40-50 deg.C in the proportion of 3000-5000 U / g substrate, stirring at constant temperature, making enzymolysis for 3-5 hours, then killing the enzyme at 95-110 deg.C for 4-6 minutes, cooling to normal temperature and freeze-drying. It produces the active peptides of reducing blood pressure and easy to obtain the active peptides with more reasonable molecular weight distribution.

Description

technical field [0001] The invention relates to a method for preparing blood pressure-lowering active peptide by step-by-step directional enzymatic hydrolysis of fish skin collagen with compound enzyme. Background technique [0002] At present, some people use soybean, casein, sardine meat, tuna meat, etc. as raw materials, and use trypsin, chymotrypsin, pepsin, thermolysin, Alcalase alkaline protease, etc. peptide. The prepared product is mainly used as an amino acid nutritional functional food. Many people in China are developing active peptides with blood pressure-lowering function using different raw materials as objects. The main problems at present are the high price of raw materials used, poor enzymatic hydrolysis effect, low activity of active peptides, and low yield. Contents of the invention [0003] The purpose of the present invention is to provide a method for producing active peptides by step-by-step directed enzymolysis of fish skin collagen with complex e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00
Inventor 曾名湧陈胜军李八方刘尊英董士远
Owner OCEAN UNIV OF CHINA
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