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Method for preparing hemolysis agent for blood cell analysis instrument use

A blood cell analyzer and production method technology, applied in the direction of individual particle analysis, particle and sedimentation analysis, preparation of test samples, etc., can solve the problems of inconvenient production and storage of hemolytic agents, achieve quality assurance, prolong storage life, The effect of prolonging life

Inactive Publication Date: 2004-11-24
上海捷瑞医用试剂有限公司
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Problems solved by technology

[0003] A hemolytic agent is needed to analyze blood with a hematology analyzer, and the major manufacturers of hemolytic agents in the world, such as American ABBOTT Company, COULTER Company, Japanese East Asia Company, and French ABX Company, produce hemolytic agents with a validity period of It is one year (see the instructions for use of the hemolytic agent), which greatly inconveniences the production and storage of the hemolytic agent, and even makes some hemolytic agents expire and become useless

Method used

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Examples

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Embodiment 1

[0015] The high-pressure reverse osmosis filter membrane is used to produce filtered water, and after treatment with ion exchange resin, water with a resistance of ρ=9MΩ / cm is placed in the mixing tank. The diameter of the blade of the mixer is two-thirds of the diameter of the mixing tank, and the motor The number is 40 rpm, add dodecyltrimethylammonium chloride, stir at 50°C for 1 hour, dissolve, cool to 20°C and then add tetradecyltrimethylammonium bromide. Stir and dissolve for 24 hours, finally add potassium cyanide and other raw materials, stir for 15 minutes, irradiate with ultraviolet light for 1 hour, filter with three-stage filters in series, each filter has a filter pore size of 02 μm, fill it, and wait for delivery.

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Abstract

The production process of hemolytic agent for hemocyte analyzer includes the following steps: water processing via filtering in high pressure reverse osmosis membrane and ion exchange with resin to obtain water with resistivity greater than 10 megaohm / cm, adding and dissolving ammonium dodecyl trimethyl chloride under stirring and cooling to 15-30 deg.c, adding ammonium tetradecyl trimethyl bride, adding potassium cyanide and other material, sterilizing, filtering and packing. The hemolytic agent has high quality and long storing life up to 2 years.

Description

technical field [0001] The invention relates to a production method of a hemolytic agent for a blood cell analyzer. Background technique [0002] It is very important for doctors to diagnose the disease and its mechanism by performing laboratory tests on the patient's blood to understand the status of the patient's red blood cells, white blood cells, and platelets. The original method of blood testing was that the doctor used a microscope to arrange slices of glass and measure the number with the naked eye. At present, blood cell analyzers are widely used to replace manual operations, which greatly improves work efficiency. [0003] A hemolytic agent is needed to analyze blood with a hematology analyzer, and the major manufacturers of hemolytic agents in the world, such as American ABBOTT Company, COULTER Company, Japanese East Asia Company, and French ABX Company, produce hemolytic agents with a validity period of It is one year (see the instructions for use of the hemoly...

Claims

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Application Information

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IPC IPC(8): G01N1/38G01N15/12G01N33/50
Inventor 苏跃新刘文义吴维杰何百钢刘树波张惜今唐恩东
Owner 上海捷瑞医用试剂有限公司
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