Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for preparation of gallic acid by co-culture

A gallic acid, co-cultivation technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of long time and low yield

Inactive Publication Date: 2004-07-28
INDIAN INST OF TECH +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] However, the prior art methods suffer from the disadvantages of long time requirements and very low reported yields

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] Isolation of tannase-producing microorganisms

[0032] Two strains of filamentous fungi Rhizopus oryzae (RO IIT RB13, NRRL-21498) and Aspergillus foetidus (GMRB013) were isolated from the soil of I.I.T., Kharagpur campus using the bait-bait technique. The isolated microorganisms were maintained on 2% malt extract agar slants.

[0033] Passaging of microorganisms on slants

[0034] 100 ml of malt extract agar (MEA) medium was sterilized. After sterilization, 5 ml of this was transferred to a sterile test tube and allowed to cool in the position where the slope was formed. After solidification, streak inoculate Rhizopus oryzae (RO IIT RB13, NRRL-21498) and Aspergillus foetidus (GMRB013) at a ratio of 1:1 on a sterile slant and culture in an incubator at 30°C for 72-96 hours.

[0035] The slant medium was then used for further manipulation or stored in a refrigerator at 4 °C.

[0036] Preparation of induction inoculum

[0037]Since tannase is an adaptive enzyme, a pre...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
humidityaaaaaaaaaa
Login to View More

Abstract

A process for the preparation of gallic acid using co-culture comprising providing a tannin-rich mixed substrate and a culture medium in fluid communication with each other, adding an induced innoculum comprising the fungi Rhizopus oryzae and Aspergillus foetidus to the substrate to obtain a fermented mass and gallic acid, extracting the gallic acid from the culture medium and the fermented mass using an organic solvent followed by evaporation of the organic solvent to obtain gallic acid.

Description

technical field [0001] The invention relates to a method for preparing gallic acid through co-cultivation. Background technique [0002] Enzymes are specific proteins of living tissue that function as biocatalysts. Enzymes are capable of performing reactions at normal temperatures and pressures that would require costly energy or not at all at elevated temperatures and / or pressures. That is, the use of enzymes in industry is increasing. [0003] A variety of microorganisms produce extracellular enzymes. They are mainly hydrolases and are primarily concerned with the degradation of macromolecules into units that can be taken up by living cells. Since microorganisms are widely used in the production of enzymes, new methods for the preparation of many important industrial chemicals are constantly being explored. [0004] New developments in the field of biotechnology have resulted in new uses for microbial enzymes. In addition to their traditional use in the food industry ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/18C12P7/42C12R1/66
CPCC12N9/18C12P7/42
Inventor R·巴纳吉G·穆克赫吉
Owner INDIAN INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products