Process for preparation of gallic acid by co-culture
A gallic acid, co-cultivation technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of long time and low yield
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[0031] Isolation of tannase-producing microorganisms
[0032] Two strains of filamentous fungi Rhizopus oryzae (RO IIT RB13, NRRL-21498) and Aspergillus foetidus (GMRB013) were isolated from the soil of I.I.T., Kharagpur campus using the bait-bait technique. The isolated microorganisms were maintained on 2% malt extract agar slants.
[0033] Passaging of microorganisms on slants
[0034] 100 ml of malt extract agar (MEA) medium was sterilized. After sterilization, 5 ml of this was transferred to a sterile test tube and allowed to cool in the position where the slope was formed. After solidification, streak inoculate Rhizopus oryzae (RO IIT RB13, NRRL-21498) and Aspergillus foetidus (GMRB013) at a ratio of 1:1 on a sterile slant and culture in an incubator at 30°C for 72-96 hours.
[0035] The slant medium was then used for further manipulation or stored in a refrigerator at 4 °C.
[0036] Preparation of induction inoculum
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