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Low temperature nitrobacter agent and use thereof

A nitrifying bacteria agent and a low-temperature-resistant technology, applied to the low-temperature nitrifying bacteria agent and its application field, can solve the problems of unsatisfactory efficiency, unsatisfactory ammonia nitrogen removal ability and efficiency, insufficient ammonia nitrogen removal efficiency and ability, etc.

Inactive Publication Date: 2004-07-07
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these bacterial agents have a certain effect on removing ammonia nitrogen and purifying seawater for cultivation, they all have obvious shortcomings. It is unbearable for domestic marine product breeding owners to add permanent dosage. At the same time, when the temperature is lower than 20 degrees Celsius, its ability and efficiency to remove ammonia nitrogen obviously cannot meet the requirements; aquarium water purification microorganisms and immobilized microorganisms are both The results of laboratory research are still far from practical application, and their efficiency and ability to remove ammonia nitrogen under the condition of less than 20 degrees Celsius are also obviously insufficient

Method used

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  • Low temperature nitrobacter agent and use thereof
  • Low temperature nitrobacter agent and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Nitrifying bacteria agent to the ammonia nitrogen removal result of simulated seawater

[0031] The prepared low-temperature nitrifying bacteria agent was inoculated into artificially prepared simulated seawater containing 5 mg / L of ammonia nitrogen at an inoculum amount of 5%, and cultured on a shaking table at 25°C to carry out the experiment of removing ammonia nitrogen. reaction can be completely removed. The result is shown in Figure 3.

Embodiment 2

[0032] Example 2 Ammonia Nitrogen Removal Experiment of Simulated Small System Operation After Filling Films

[0033] After the low-temperature nitrifying bacteria agent is formed on the carrier filler according to the operation in the embodiment, the test of removing ammonia nitrogen is carried out in the simulated small system. The circulating seawater of the simulated small system is 3m 3 , the effective volume of the bioreactor is 0.300m 3 , which is 1 / 10 of the circulating seawater. The filler in the bioreactor is a natural plant carrier filler, and the filling degree reaches a specific surface area of ​​100m 2 / m 3 , At the end of October and the beginning of November, the removal experiments of different ammonia nitrogen concentrations were carried out, and the results are listed in Table 1.

[0034] date

[0035] It can be seen from Table 1 that when the water temperature is low, the removal efficiency of the ultra-low concentration of ammonia nitrogen in...

example 3

[0036] In the scallop nursery of Qingdao Jinying Ocean Technology Development Co., Ltd., see the biological treatment system figure 2 . The on-site circulating seawater seedling cultivation test was carried out, and circulating seawater was used throughout the seedling cultivation cycle (about 2 to 3 weeks), and the seedlings were poured into the pond once in about 4 days during the period. During this period, the ammonia nitrogen in the influent and effluent water of the biological treatment system was detected. The results showed that under the condition of water temperature of about 17°C, the concentration of ammonia nitrogen in the influent water was 50-65 μg / L, and the concentration of ammonia nitrogen in the effluent was maintained below 30 μg / L. The requirements ensured the smooth progress of scallop seedlings.

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Abstract

A low-temp nitrating bacteria preparation for removing NH3-N and COD from the seawater used in marine farm is prepared from Nitrobacter hamburgensis (AS 1.2729), Nitrobacter winogradskyi (AS 1.2730), Nitrobacter vulgaris (AS 1.2775), Bacillus cereus (AS 1.230), Bacillus licheniformis (AS 1.518) and saccharomyces cerevisiae (AS 2.412) through culturing, and proportional mixing.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, specifically, it relates to a low-temperature resistant nitrifying bacterial agent, and the low-temperature nitrifying bacterial agent relates to Nitrobacter hamburgensis AS1.2729, Nitrobacter winogradskyi AS 1.2730, and Nitrobacter vulgaris AS 1.2775 , Bacillus cereus AS 1.230, Bacillus licheniformis AS 1.518, and Saccharomyces cerevisiae AS 2.412, a total of six strains. After these strains are cultured, they are composed of low-temperature resistant nitrifying bacteria according to the same volume ratio of the culture It has the effect of removing ultra-low concentration of ammonia nitrogen at a lower temperature, and also has a certain effect of removing COD and BOD. Adapted to the corresponding bioreactor, it is suitable for the removal of ammonia nitrogen and COD in general mariculture and high-density culture in industrialized circulating seawater. technical background ...

Claims

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Application Information

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IPC IPC(8): A01K61/00C02F1/58C02F3/34C12N1/20
CPCY02A40/81
Inventor 刘志培贾省芬黄志勇刘双江
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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