Method for producing zygotic appearance cotyledon type pine embryo in high yield by using culture medium containing diose and glucose

A medium, glucose technology, applied in the field of plant embryo production, to achieve the effect of high germination rate and quality

Inactive Publication Date: 2004-02-25
WEYERHAEUSER CO
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But a persistent problem is promoting the efficient formation of cotyledon-type pine embryos that can germinate at high frequencies to produce pine plants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing zygotic appearance cotyledon type pine embryo in high yield by using culture medium containing diose and glucose
  • Method for producing zygotic appearance cotyledon type pine embryo in high yield by using culture medium containing diose and glucose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This example shows a representative method of the invention for producing taeda pine (Pinus taeda) populations, cotyledon-type embryos, and germination of the embryos.

[0045] Large gametophytes containing zygotic embryos were removed from seeds 4-5 weeks after fertilization. The seed coat was removed, but the embryo was not further separated from the surrounding gametophyte other than the excision of the nucellar end. Store the cones at 4°C until use. Immediately before the removal of immature embryos, the seeds undergo a sterilization process: initial washing and detergent treatment, followed by 15% H 2 o 2 Sterilize for 10 minutes. Wash the explants thoroughly with sterile distilled water after each treatment.

[0046] Table 1 lists the composition of representative media used for taeda pine embryogenic tissues.

[0047] Loblolly pine medium composition

BM

BM1

BM2

BM3

BM4

BM5

BM6

BM7

Salt (mg / L)

...

Embodiment 2

[0059] This example shows the effect on embryo development and germination frequency of culturing somatic embryos of Pine taedablolly on development medium containing 2.5% maltose and 1% glucose.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides methods for producing cotyledonary pine embryos. The methods of the invention each include the step of culturing embryogenic pine tissue in, or on, a medium comprising a disaccharide and glucose to yield cotyledonary pine embryos, wherein the disaccharide and glucose are each present in the medium at a concentration of less than 3 percent. The present invention also provides cotyledonary pine embryos prepared by a method of the invention.

Description

technical field [0001] The present invention relates to a method for producing plant embryos outside the plant and producing plants from the plant embryos arbitrarily. Background technique [0002] Demand for pine continues to rise for the manufacture of wood-based products. A proposed solution to this problem is to identify individual trees with desirable characteristics such as fast growth and obtain a large number of genetically identical clones of the superior tree through somatic cell cloning. These clones can be bred to obtain pine forests, or entire forests, with desired properties. [0003] A method for cloning pine trees is to process isolated living pine tree tissues in vitro under conditions that promote the formation of pine embryos, and then obtain whole plants from the treated tissues. Ex vivo pine tissue can be cultured in the presence of one or more auxins and / or cytokinins to promote the formation and proliferation of embryogenic tissue, followed by cultur...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 巴莫德·K·古普塔黛安娜·霍尔姆斯特伦邦尼·拉森
Owner WEYERHAEUSER CO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap