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Method for detecting starch content in ash tree flower polysaccharide

A technique for determination of polysaccharides from Grifola frondosa, which is applied in the direction of chemical analysis by titration, can solve problems such as the uncertainty of accurate detection in quality control, and achieve stable and reliable determination results, small coefficient of variation, and strong operability. Effect

Inactive Publication Date: 2004-02-11
LAIYANG AGRI COLLEGE
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Problems solved by technology

However, there is no ideal method for the determination of the starch content of inactive components in Grifola frondosa polysaccharides, but the national standard "GB / T 5009.9-1985 Determination of starch in food" is used for determination; In addition to the content of the inactive component starch in the flower polysaccharide, it also contains the content of the active polysaccharide of Grifola frondosa, which makes the quality control of the active polysaccharide product of Grifola frondosa and the accurate detection of the active polysaccharide content of Grifola frondosa produce a great deal uncertainty

Method used

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Experimental program
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Embodiment 1

[0024] 1) Removal of fat-soluble components: Take the dried and ground polysaccharide samples (No. 1), weigh 1.0727 grams, 1.1028 grams, 1.1509 grams, 0.948 grams, 1.1813 grams, and 1.0481 grams, respectively, and place them in 6 centrifugal cups , respectively add 30ml of ether, shake fully for 12 minutes, centrifuge at 4500 rpm for 8 minutes, discard the supernatant, then add 30ml of ether, and repeat the centrifugation for 3 times;

[0025] 2) Remove soluble carbohydrate interfering substances: add 30 ml of 80% ethanol at 57°C to the above-mentioned centrifuge cup after discarding the supernatant, shake fully for 15 minutes, centrifuge at 5000 rpm for 10 minutes, discard the supernatant, Repeat this centrifugation 3 times; then add 30ml of 50°C distilled water to the centrifuge cup after discarding the supernatant, shake in a constant temperature water bath at 50°C for 60 minutes, centrifuge at 5000 rpm for 5 minutes, discard the supernatant, Then add 30ml of distilled wate...

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Abstract

The present invention is the method of detecting starch content in ash tree flower polysaccharide product. The detection includes the steps of eliminating liposoluble component, eliminating soluble saccharide as interference matter, enzyme decomposing starch, detecting starch, processing enzyme hydrolyzed starch liquid, Fehling's process of determining reducing sugar content, etc. The method ofthe present invention is suitable for operation, has stable and reliable determined result, small variation coefficient of determined result, less error, high repeatability and other advantages.

Description

(1) Technical field [0001] The invention relates to a method for determining the polysaccharide extract of Grifola frondosa frondosa, in particular to a method for quantitatively determining the starch content of inactive components in the polysaccharide extract of the deep layer liquid fermentation broth of Grifola frondosa. (2) Background technology [0002] The polysaccharide extract of Grifola frondosa submerged liquid fermentation liquid mainly includes the active component polysaccharide and the inactive component polysaccharide-starch; wherein, the quantitative determination of the inactive component starch is important for the quality control of the active polysaccharide product of Grifola frondosa and the production process of the polysaccharide product. Definitely is very necessary. However, there is no ideal method for the determination of the starch content of inactive components in Grifola frondosa polysaccharides, but the national standard "GB / T 5009.9-1985 Det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/16
Inventor 隋方功宋爱荣张玉娜吕银燕
Owner LAIYANG AGRI COLLEGE
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