HLA binding peptides and their uses

A technique of combining motifs and compositions, applied in the field of HLA-binding peptides and their uses, can solve the problems that vaccines or therapeutic agents have not been developed

Inactive Publication Date: 2003-10-29
埃皮缪纳股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Despite advances in the field, no useful human peptide-based vacci

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0155] Isolation of class I antigens is carried out using the method described in the above-mentioned related application. The naturally processed peptides are then isolated and sequenced using the methods described herein. Determine an allele-specific motif and various algorithms, and perform a set of quantitative binding experiments.

[0156] The various motifs identified above for the amino acid sequence of HLA-A2.1 alleles from several antigens were used to analyze whether these motifs exist. Table 3 provides the results of these studies. The letter "J" stands for norleucine.

[0157] Some of the above embodiments are provided to illustrate the present invention, not to limit its scope. Other variants of the present invention are easily met by those of ordinary skill in the art, and they are included in the appended claims. All publications, patents and patent applications cited herein are incorporated herein by reference.

[0158] table 3 Peptides A...

Embodiment 2

[0406] Identification of immunogenic peptides

[0407] The above motifs identified from the amino acid sequences of various class I MHC alleles of various pathogens and tumor-related proteins are used to analyze whether these motifs exist. Screen as described in the relevant application. Table 12 provides the search results for these antigens.

[0408] Table 12 Sequence of peptide AA Source A * 0301 A * 110128.0719 10 ILEQWVAGRK HDV.nuc.16 0.0170 0.001228.0727 10 LSAGGKNLSK HDV.nuc.115 0.0097 0.01501259.02 11 STDTVDTVLEK Flu.HA.29 0.0001 0.06701259.04 9 GIAPLQLGK Flu.HA.63 0.6100 0.20001259.06 10 VTAACSHAGK Flu.HA .149 0.0380 0.04901259.08 9 GIHHPSNSK Flu.HA.195 0.1300 0.01401259.10 10 RMNYYWTLLK Flu.HA.243 2.5000 2.30001259.12 11 ITNKVNSVIEK Flu.HA.392 0.0200 0.06701259.13 11 KMNIQFTAVGK Flu.HA.402 0.0280 0.00921259.14 9 NIQFTAVGK Flu.HA.404 0.0017 0.03301259.16 11 AVGKEFNKLEK Flu.HA.409 0.0210 0.04601259.19 11 KVKSQLKNNAK Flu.HA.465 0.0470 0.00311259.20 1...

Embodiment 3

[0427] Identification of immunogenic peptides

[0428] Some supercoiled motifs identified as B7 in the related application are used to analyze whether these motifs exist in the sequences of various pathogens and tumor-related proteins. Screen as described in the relevant application. Table 13 provides the search results for these antigens.

[0429] Table 13

[0430] Peptide sequence source

[0431] 40.0013 SPGLSAGI CEA.680I8

[0432] 40.0022 KPYDGIPA Her2 / neu.921

[0433] 40.0023 KPYDGIPI Her2 / neu.921I8

[0434] 40.0050 APRMPEAA p53.63

[0435] 40.0051 APRMPEAI p53.63I8

[0436] 40.0055 APAAPTPI p53.76I8

[0437]Table 13 (Continued) Source of peptide sequence 40.0057 APTPAAPI p53.79I840.0059 TPPAAPAPI p53.81I840.0061 APAPAPSI p53.84I840.0062 SPALNKMF p53.12740.0063 SPALNKMI p53.127I840.0117 SPSAPPHRI CEA.3I940.0119 PPHRWCIPI CEA.7IGRE940.0120 CEA.9240.0156 MPNQAQMRILI Her2 / neu.706I1040.0157 MPYGCLLDHVI Her2 / neu.801I1040.0161 APPHRWCIPW CEA.640.0162 APPHRWCIPI CEA.6...

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Abstract

The present invention provides means and methods for selecting immunogenic peptides and immunogenic peptides capable of specifically binding to glycoproteins encoded by HLA alleles and inducing T cell activation in T cells restricted by the alleles combination. These peptides can be used to elicit an immune response against the desired antigen.

Description

[0001] Reference to related applications [0002] The application for the present invention is a partial continuation application of USSN 08 / 205,713 (filed on March 4, 1994). This application also relates to USSN09 / 017,735, USSN08 / 753,622, USSN08 / 822,382, USSN60 / 013,980, USSN08 / 589,108, USSN08 / 454,033, USSN08 / 349,177, USSN08 / 073,205 and USSN08 / 027,146. The present application also relates to USSN09 / 017,524, USSN08 / 821,739, USSN60 / 013,833, USSN08 / 758,409, USSN08 / 589,107, USSN08 / 451,913 and USSN08 / 347,610, USSN08 / 186,266, USSN08 / 159,339, USSN09 / 116,061, USSN08 / 103,396, USSN08 / 027,746 and USSN07 / 926,666. The present application also relates to USSN09 / 017,743, USSN08 / 753,615, USSN08 / 590,298, USSN08 / 452,843, USSN09 / 115,400, USSN08 / 344,824 and USSN08 / 278,634. The present application also relates to USSN08 / 197,484 and USSN08 / 815,396. This article incorporates all the above applications as a reference. Background of the invention [0003] The present invention relates to compositions and ...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K38/00A61K38/04A61K38/08A61P31/12A61P35/00A61P37/04A61P43/00C07K7/00C07K7/04C07K7/06
CPCA61K38/00A61K39/00A61K2039/57A61P31/12A61P35/00A61P37/04A61P43/00C07K7/06
Inventor A·塞特J·悉尼W·M·卡斯特S·索思伍德
Owner 埃皮缪纳股份有限公司
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