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Medical use and medicinal preparation of trichonema in preventing human and animals from diseases by immunologic inadequacy

A technology of low function and animal immunity is applied to the medical use of Trichoderma nematode in preventing and treating diseases caused by low immune function of humans and animals. To achieve a wide range of effects, easy to use, good effect

Inactive Publication Date: 2003-09-03
MILITARY SUPPLIES UNIV THE CHINESE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the long-term biological evolution process, Trichinella spiralis (Owen 1835) survives in humans or animals for short, and produces many beneficial natural antigenic substances. Although the structure and function of these complex substances have not yet been clarified, But it does have the effect of non-specifically enhancing the immune function of humans or animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: preparation of Trichinella nematode cell seedlings:

[0030] Aseptically collected 5000 Trichinella nematodes, put them into a mortar, crushed the worms, digested them with 0.25% trypsin for 30 minutes to make a cell suspension, washed them three times with PH7. The sediment was made into a suspension, which was divided into three 30ML cell culture bottles pre-coated with collagen, plugged with rubber stoppers, cultured in a 37°C incubator, and discarded when the cells basically grew into a single layer. The supernatant was washed once with GNH (that is, the calcium salt, magnesium salt and phosphate were removed from the formula of Hanks’ solution), soaked in pre-warmed 0.25% trypsin solution for 20 min to desalt the cells, then centrifuged, and discarded the supernatant. Add 6ml of new culture solution, mix well, divide into two bottles, continue to culture, and continuously subculture for 30 generations, observe the growth rate curve and cell colony for...

Embodiment 2

[0031] Embodiment 2: Preparation of attenuated seedlings of Trichinella nematode

[0032] The rats artificially infected with Trichinella trichinella were put to death, and the muscle was taken and crushed with a meat blender, and put into digestive juice (pepsin 0.5g, hydrochloric acid 0.7ml, water 100ml), after digestion, the worm body was collected, and the worm body was Placed in PBS for Co 60 Save after irradiation.

Embodiment 3

[0033] Embodiment 3: chemical extraction method of Trichinella trichinellae biologically active substance

[0034] Crush 100,000 worms and weigh them, put them into a round bottom flask and add an appropriate amount of 70% ethanol to soak overnight, then heat and reflux for 2-3 hours, filter, concentrate the ethanol extract, and continue to use 70% ethanol to heat and reflux for 4-5 hours for the remaining residue. Once, combine the ethanol concentrate, boil the remaining residue with water twice, concentrate the boiled liquid, merge into the ethanol concentrate, dry and weigh the residue for later use. Take part of the concentrated ethanol solution and add an appropriate amount of petroleum ether to extract it several times, and the remaining residue is extracted several times with ethyl acetate. Finally, 5 parts of ethanol total extract, ethanol total extract residue, petroleum ether extract, ethyl acetate extract and residue were obtained for future use.

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PUM

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Abstract

An application of Trichonematidae nematode in preventing and treating the diseases caused by human or animal's lowimmunologic function is disclosed. Its relative medicinal preparations include spirotrich nematode cell vaccine, spirotrich nematode atenuator vaccine and bioactive substance of spirotrich nematode.

Description

Technical field: [0001] The present invention relates to the medical application of Trichinella nematodes, especially discloses the medical application of a kind of Trichinella nematodes in the prevention and treatment of diseases caused by hypoimmunity of humans and animals, and provides corresponding pharmaceutical preparations, which belongs to biopharmaceutical technology field. Background technique: [0002] For many years, people have always believed that parasites are a kind of pests that live in human or animal bodies, but from the perspective of dialectical materialism, parasites should also have their beneficial and usable side. In the long-term biological evolution process, Trichinella spiralis (Owen 1835) survives in humans or animals for short, and produces many beneficial natural antigenic substances. Although the structure and function of these complex substances have not yet been clarified, But it does have the effect of non-specifically enhancing the immune...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/64A61K39/00A61P33/00A61P37/04
Inventor 张西臣李建华尹继刚
Owner MILITARY SUPPLIES UNIV THE CHINESE PLA
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