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Double-parameter cell period analysis method

A cell cycle and analysis method technology, applied in the field of basic medicine, can solve the problems of increasing the probability of incorrect cell cycle analysis and the difficulty of taking only a small number of cell samples, so as to save the amount of sample cells, avoid blindness, The effect of strong dosage

Inactive Publication Date: 2003-05-14
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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Problems solved by technology

However, in order to conduct a more detailed analysis of the cell cycle, people have to analyze a single type of cyclin one by one, which cannot avoid the error between multiple experiments, which leads to the inaccuracy of the cell cycle analysis Increased probability
Therefore, in clinical and laboratory analysis of cell samples, it is difficult to meet the medical requirement of taking only a small number of cell samples and distinguishing them in more detail by using the above-mentioned method of detecting each type of cell cycle protein one by one.

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  • Double-parameter cell period analysis method
  • Double-parameter cell period analysis method
  • Double-parameter cell period analysis method

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Embodiment Construction

[0027] Specific embodiments of the present invention are described in further detail below in conjunction with the accompanying drawings;

[0028] Fromfigure 1 As can be seen from the histogram of cyclin DNA content shown, the cells can only be distinguished into G by ordinary flow cytometry 0 / G 1 phase, S phase, G 2 Three cell populations in / M phase.

[0029] From figure 2 The two-dimensional contour map of the expression of cyclin E shows that the cells can only be distinguished into G cells by Cyclin E / DNA dual-parameter flow cytometry. 0 period, early G 1 period, late G 1 phase, S phase, G 2 Five cell populations in / M phase.

[0030] From image 3 The two-dimensional contour map of cyclin A expression shown shows that Cyclin A / DNA dual-parameter flow cytometry can only distinguish cells into G 0 / G 1 phase, S phase, G 2 Phase and M phase four cell populations.

[0031] Taking the analysis of acute T lymphocytic leukemia cells (MOLT-4 cells) as an example, t...

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Abstract

The double-parameter cell cycle analysis method separates cell cycle into six fine parts. Mouse anti-human cell cycle protein E monoclonal antibody, mouse anti-human cell cycle protein A monoclonal antibody and calf serum albumin liquid are mixed to constitute mixed antibody, and the mixed antibody and the mixed liquid of propyldine iodide and ribonuclease are utilized to dye various cells, including normal cell and tumor cell, to be analyzed, the cell is fluorescent detected with flow cell instrument to obtain the 2D cell cycle protein E+A contour line diagram to show six colonies in Go cycle, early G1 cycle, late G1 cycle, S cycle, G2 cycle and M cycle. The said method has less detection error and saving in cell amount and has guide significance in the analysis and treatment of tumor.

Description

technical field [0001] The invention relates to the analysis and research of cells in the field of basic medicine, in particular to a double-parameter cell cycle analysis method. Background technique [0002] The analysis and research on cells in the field of basic medicine started more than one hundred years ago. In 1879, Flemming W. observed living cells through an optical microscope, and the cell cycle could be divided into mitotic interphase and mitotic phase. This is the germination of the cell cycle analysis method, which is simple and roughly conceivable. With the continuous in-depth research on cells and the continuous development and updating of detection techniques, in 1969, Vandilla MA. used flow cytometry to analyze the deoxyribonucleic acid (DNA) in cells, and obtained it through flow cytometry. The deoxyribonucleic acid content histogram (DNA content histogram) can divide the cell cycle into G 0 / G 1 phase, S phase, G 2 Three cell po...

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Application Information

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IPC IPC(8): C12Q1/02G01N33/53G01N33/533G01N33/577
Inventor 龚建平覃吉超陶德定冷彦申漫里冯永东高纯余源
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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