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Recombinant herpesvirus carrying antivascular and antiendothelial cell factors fusion gene

An endothelial cytokine and fusion gene technology, applied in the field of recombinant herpes virus, can solve the problems of limited ability to lyse tumors and affect the therapeutic effect, etc.

Inactive Publication Date: 2003-02-19
罗益(无锡)生物制药有限公司
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Problems solved by technology

[0003] In 1991, for the first time abroad, the recombinant HSV-1 virus tk(-) HSV-1 with the deletion of the tk gene was used to prove that the tk(-) HSV-1 virus can selectively To kill tumor cells without causing serious brain nerve tissue damage, because tk(-)HSV-1 virus has limited ability to lyse tumors, it cannot produce biological diffusion effect on tumors, which affects the clinical treatment effect

Method used

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  • Recombinant herpesvirus carrying antivascular and antiendothelial cell factors fusion gene
  • Recombinant herpesvirus carrying antivascular and antiendothelial cell factors fusion gene
  • Recombinant herpesvirus carrying antivascular and antiendothelial cell factors fusion gene

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Embodiment Construction

[0020] VAE is caused by wild HSV-1 virus F (see figure 1 ) through genetic engineering, the wild strain virus was first isolated in New York in 1922 from patients infected with HSV-1 virus. The genetic characteristics of VAE are: 1. The two copies of the ICP34.5 gene located in the repetitive gene region have been partially deleted and lost function; 2. The ICP6 gene located in the long non-repeated gene region has also been partially deleted, and The fusion gene (Endo: angio) of human Endostatin (Cirri, Donnini et al.1999) and Angiostatin (O'Reilly, Holmgrenet al.1994; O'Reilly 1997) was inserted; 3. Two ICP4 genes located in the duplication The copy is temperature sensitive and loses activity at 39 degrees Celsius. The above features are the same as G207 except that the gene carried by the ICP6 gene region is different, except that the β-galactosidase of Escherichia coli is inserted into the ICP6 gene region of G207. Therefore, VAE and G207 are basically the same, that is,...

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Abstract

A recombinant herpesvirus carrying the endostatin and angiostatin fused gene for effectively treating colloima features that its original virus HSV-1 contains a long nonrepetitive region (UL) and a short one (US). After the ICP6 gene in the UL is partly removed, the human endostatin and angiostatin is inserted to obtain the fused gene.

Description

technical field [0001] The invention relates to biotechnology, in particular to a recombinant herpes virus carrying anti-vascular and anti-endothelial cytokine fusion genes. technical background [0002] Herpes simplex virus type 1, or HSV-1 for short, can infect many different types of human and animal cells, including epithelial and endothelial cells. After the wild-type virus infects non-neural cells in vivo, it usually replicates in large quantities in the cells and causes cell lysis, but when it infects nerve cells, it usually only has limited replication or no replication and quickly enters the latent period. Since the damage of HSV-1 to nerve cells is usually smaller than that of non-nerve cells, as early as the 1960s, some people tried to use it to treat malignant glioblastoma. Because wild-type HSV-1 is more toxic after all, this experiment could not be carried out. [0003] In 1991, for the first time abroad, the recombinant HSV-1 virus tk(-) HSV-1 with the delet...

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Application Information

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IPC IPC(8): C12N7/01C12N15/62
Inventor 瞿伯荣
Owner 罗益(无锡)生物制药有限公司
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