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Method for inhibiting macrophage infiltration using monoconal anti-alpha-D-antibodies

A monoclonal antibody, macrophage technology, applied in other fields, can solve the problem of indistinguishable

Inactive Publication Date: 2002-06-05
ICOS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Method for inhibiting macrophage infiltration using monoconal anti-alpha-D-antibodies
  • Method for inhibiting macrophage infiltration using monoconal anti-alpha-D-antibodies
  • Method for inhibiting macrophage infiltration using monoconal anti-alpha-D-antibodies

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Try to detect canine alpha TM1 homologue of

[0056] In an identification dog alpha TM1 In an attempt of the human homologue, the test was performed against the canine α TM1 Cross-reactivity of the specific monoclonal antibody Ca11.8H2 [Moore et al., supra] on human peripheral blood leukocytes. In the presence of 0.1% sodium azide, cell preparations (usually 1×10 6 cells) were incubated on ice with undiluted hybridoma supernatant or purified mouse IgG negative control antibody (10 μg / ml). Binding of monoclonal antibodies was detected by subsequent incubation with 6 μg / ml FITC-conjugated horse anti-mouse IgG (Vector Laboratories, Burlingame, CA). Stained cells were fixed with 2% w / v paraformaldehyde in phosphate buffered saline (PBS) and analyzed with a Facstar Plus fluorescence activated cell sorter (Becton Dickinson, Mountain View, CA). Typically, 10,000 cells are analyzed with logarithmic amplification of fluorescence intensity.

[0057] The result...

Embodiment 2

[0060] Affinity purified canine alpha TM1 for N-terminal sequencing

[0061] Affinity purified canine alpha TM1 To determine the N-terminal amino acid sequence for the design of oligonucleotide probes / primers. In short, the anti-alpha TM1 Monoclonal antibody Ca11.8H2 with Affigel  10 Chromatography resin (BioRad, Hercules, CA) coupled to separate proteins by specific antibody-protein interactions. Antibodies were conjugated to the resin at a concentration of approximately 5 mg antibody / ml resin following the protocol suggested by BioRad. After the conjugation reaction, excess antibody was removed and the resin was blocked with three volumes of 0.1 M ethanolamine. The resin was then washed with 30 column volumes of phosphate buffered saline (PBS).

[0062] 25 grams from a single dog spleen 23 grams of a single dog spleen was homogenized in 250 ml of 25 mM Tris-HCl pH 8.0 buffer containing 0.32 M sucrose and protease inhibitors. Nucleic acids and cellular ...

Embodiment 3

[0077] Large scale affinity purification of canine alpha TM1 for in-house sequencing

[0078] To provide additional amino acid sequences for primer design, purified canine α TM1 For in-house sequencing. Three frozen spleen sections (about 50 g each) and frozen cells from two partial spleens from adult dogs were used to generate proteins for in-house sequencing. 50 g of spleen was homogenized in 200-300 ml of boric acid buffer with a Waring blender. The homogenized material was diluted with 1 volume of buffer containing 4% NP-40, and the mixture was stirred gently for at least 1 hour. Large debris in the resulting lysate was removed by centrifugation at 200Og for 20 minutes, then filtered through either a Corning (Corning, NY) prefilter or a Corning 0.8 micron filter. The lysate was further clarified by passing through a Corning 0.4 micron filter system.

[0079] Spleen lysate and antibody-conjugated Affigel as described in Example 2  10 Resins were mixed in 1...

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Abstract

Methods to inhibit inflammation and macrophage infiltration following spinal cord injury are disclosed along with methods to modulate TNFalpha release from cells expressing alphad are disclosed.

Description

[0001] This application is a continuation-in-part of pending U.S. Patent Application Serial No. 09 / 193,043, filed November 16, 1998, which is a continuation-in-part of pending U.S. Patent Application Serial No. 08 / 943,363, filed October 3, 1997 , U.S. Patent Application Serial No. 08 / 943,363 is a continuation-in-part of U.S. Patent Application Serial No. 08 / 605,672 filed on February 22, 1996 and granted as U.S. Patent 5,817,515 on October 6, 1998, U.S. Patent Application Serial No. 08 / 605,672 is a continuation-in-part of U.S. Patent Application Serial No. 08 / 362,652 filed on December 21, 1994 and granted as a continuation-in-part of U.S. Patent 5,766,850 filed on August 5, 1994 U.S. Patent Application Serial No. 08 / 286,889 filed on November 28, 1995 and granted as a continuation-in-part of U.S. Patent No. 5,470,953, U.S. Patent Application Serial No. 08 / 286,889, which in turn was filed on December 23, 1993, and U.S. Patent Application Serial No. 08 / 173,497 and was granted Augus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C12N5/12G01N33/566G01N33/48A61K38/00A61K39/395A61P1/00A61P3/10A61P9/10A61P11/00A61P11/06A61P11/16A61P19/02A61P25/00A61P31/00C07K14/705C12N5/10C12N15/02C12N15/09C12N15/12C12P21/08C12Q1/68C12R1/91G01N33/53G01N33/577G01N33/68
CPCC07K2319/00C07K16/2845A01K2217/05G01N33/6893A61K38/00C07K14/70553A61K2039/505C12Q1/6818G01N2333/70553A61P1/00A61P11/00A61P11/06A61P11/16A61P19/02A61P25/00A61P31/00A61P9/10A61P3/10
Inventor M·W·加拉廷M·范德维伦
Owner ICOS CORP
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