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Dendrobe protocorm hormoneless cultivation method

A cultivation method and protocorm technology are applied in the field of cultivation of Chinese herbal medicine dendrobium, can solve the problems of few dendrobium resources, long growth cycle of test-tube seedlings, reduced yield and the like, and achieve the effects of shortening growth cycle, fast reproduction and short growth cycle

Inactive Publication Date: 2007-08-01
HEFEI UNIV OF TECH
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Problems solved by technology

[0003] For a long time, the dendrobium in circulation in the market is mainly collected wild, and the output is decreasing day by day. Coupled with the special ecological conditions and the deteriorating environment, the resources of dendrobium are becoming less and less. According to statistics, the annual dry cargo weight of Dendrobium huoshanense in Huoshan County, its main production area, is less than 1kg. Therefore, the preservation of rare and valuable medicinal resources of Dendrobium huoshanense and the realization of its sustainable development and utilization still need to be solved
[0004] At present, the research on medicinal Dendrobium including Dendrobium Huoshanense mainly focuses on the rapid propagation of test-tube plantlets. However, the growth cycle of Dendrobium test-tube plantlets is long, usually about 8 months. On the other hand, the problem of low success rate of transplanting has always been It has not been well resolved, seriously restricting the development of modern Chinese medicine enterprises

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0028] Take the wild Dendrobium huoshanense as an example below to illustrate the cultivation method of the present invention.

[0029] Get wild Huoshan Dendrobium seeds (collected from Dabie Mountain, Huoshan County, Anhui Province) and sterilize them with 1% sodium hypochlorite solution for 60 minutes, rinse them with sterile double distilled water for 3 times, inoculate them in a 100ml Erlenmeyer flask filled with 20ml of solid MS medium, and place them at 25 Light at ±2°C, the light condition is 30umol m -2 the s -1, 16h / day, 4-6cm long sterile test tube seedlings were obtained after induction for 7 months. Solid MS medium consists of the following components: 1900mg L -1 Potassium nitrate, 1650mg·L -1 Ammonium nitrate, 170mg·L -1 Potassium dihydrogen phosphate, 370mg·L -1 Magnesium sulfate heptahydrate, 332.25mg·L -1 Calcium chloride, 16.9mg·L -1 Manganese sulfate monohydrate, 8.6mg·L -1 Zinc sulfate heptahydrate, 6.2mg·L -1 Boric acid, 0.83mg·L -1 Potassium iod...

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Abstract

A method for culturing the protocorm of dendrobium without hormone includes such steps as providing the aseptic test-tube plantlet of dendrobium, inducing, inducing protocorm, naturalizing protocorm, reproducing protocorm, and synthesizing polyose, which can promote the generation of cell interferon (IFN gamma) and tumor necrosis factor (INF alpha).

Description

technical field [0001] The invention relates to a cultivation method of the Chinese herbal medicine dendrobium. Background technique [0002] Medicinal Dendrobium (Dendrobium), as a precious Chinese herbal medicine, was first seen in "Shen Nong's Materia Medica". ", "Compendium of Materia Medica", "Compendium of Materia Medica" and "Supplements to Compendium of Materia Medica" and other herbal medicines of all dynasties are also highly praised. . It is widely used in clinical and traditional Chinese medicine compound. It contains multiple active ingredients such as dendrobium polysaccharides and alkaloids. Modern pharmacological studies have shown that medicinal dendrobium has a significant effect on enhancing the body's immunity and inhibiting tumor growth. [0003] For a long time, the dendrobium in circulation in the market is mainly collected wild, and the output is decreasing day by day. Coupled with the special ecological conditions and the deteriorating environment,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 罗建平姜绍通查学强石玮
Owner HEFEI UNIV OF TECH
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