Method for breeding hybridized seed of stevia rebaudinum

A technology for hybrid seeds and cultivation methods, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of poor quality of stevia, high cost of seedlings, difficult to popularize, etc., so as to reduce the cost of seedlings and cultivation. , the effect of easy promotion

Inactive Publication Date: 2001-12-26
青岛创升生物科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

There are many varieties of stevia in the market, most of which are sexual old varieties, and the sexual old varieties are propagated through self-breeding. Due to the continuous degradation of the quality of the old sexual stevia varieties, the content of steviolbioside A in stevioside is low , only accounts for 20% of the total weight of stevia, and the quality of stevia produced by this variety is poor; there is also a cutting stevia variety on the market. Because the cutting varieties are male sterile, they cannot propagate themselves through breeding, but can only propagate through cuttings, and cutting propagation is laborious, and the cost of cutting seedlings is about 400 yuan per mu, and the cost of raising seedlings is high. The survival rate is unstable and difficult to promote, and the output of the above two kinds of stevia dry leaves is 150-200 kg per mu, the stevioside content in the dry leaves accounts for about 10% of the total weight of the dry leaves, and the stevioside yield is 15-20 kg per mu ,Low Yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Sow sexual stevia seeds in one mu of land. When the sexual stevia seedlings grow 4 to 6 pairs of true leaves, remove the seedlings and keep 500 sexual stevia plants. At the same time, 4 to 6 pairs will grow out of the seedlings 6,500 cuttage stevia seedlings of true leaves were moved into hybrid planting, the ratio of sexual stevia and cutting stevia hybrid plants was 1:13, the total number of sexual stevia and cutting stevia hybrid plantings was 7,000, and the cutting stevia varieties were marked Planting location, after seeding, collect the cuttings Stevia variety seeds, the yield of hybrid seeds is about 6 kg per mu.

Embodiment 2

[0015] First grow sexual stevia and cutting stevia seedlings, and transplant 1500 sexual stevia seedlings with 4 to 6 pairs of true leaves and cutting stevia seedlings with 4 to 6 pairs of true leaves in an acre of land at the same time 7,500 plants, the ratio of sexual stevia and cutting stevia hybrid plants is 1:5, and the total number of sexual stevia and cutting stevia hybrid plants is 9,000. After seeding, according to the appearance difference between cutting stevia and sexual stevia , to collect the seeds of cutting stevia varieties, and the yield of hybrid seeds is about 10 kg per mu.

Embodiment 3

[0017] First grow the sexual stevia and cutting stevia seedlings, transplant and grow 4 to 67,500 plants in one mu of land at the same time, and plant the sexual stevia seedlings and cutting stevia seedlings alternately, and the sexual stevia and cutting stevia The ratio of the number of hybrid plants is 1:3, and the total number of hybrid plants of sexual stevia and cutting stevia is 10,000. After seeding, the seeds of the cutting stevia varieties are collected separately, and the yield of hybrid seeds is about 9 kg per mu.

[0018] In the present invention, after the seedlings of the cuttage stevia varieties and sexual stevia varieties are raised, they can grow normally under normal natural conditions, can carry out pollen transfer under normal natural conditions, and obtain hybrid stevia seeds after seeding.

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Abstract

A method for breeding hybridized seed of stevia rebaudinum includes hybridizing between the stevia rebaudinum cutting as female parent and sexual stevia rebaudinum as male parent and collecting the seeds from stevia rebaudinum cutting. Its advantages are low cost, high content of rebaudioside A and high output of stevioside.

Description

technical field [0001] The invention relates to a method for cultivating stevia hybrid seeds, in particular to a method for cultivating stevia hybrid seeds by crossing cutting stevia varieties and sexual stevia varieties. Background technique [0002] Stevia is the raw material for the production of stevioside, which is extracted from the leaves of Stevia rebaudiana and is composed of stevioside (Stevioside), stevioside A (Rebaudioside A), stevioside C (Rebaudioside C) and duoside A ( The mixture composed of Dulcoside A), the main components of stevioside are stevioside A and stevioside. The sweetness of stevioside is 200-400 times that of sucrose, and its calories are only 1 / 300 of that of sucrose. It can be widely used in beverages, food, dairy products, canned food, condiments, alcohol, cosmetics, pharmaceuticals and other industries, so planting Stevia has a broad market prospect. The main quality of stevioside is determined by stevioside and st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 孙景文
Owner 青岛创升生物科技有限公司
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