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Useful properties of a bee venom protein and gene encoding same

A residue, amino acid technology, applied in the field of useful properties of melittin and the gene encoding this melittin, can solve the problem of inability to reverse the disease course and so on

Inactive Publication Date: 2001-09-19
PAN PACIFIC PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The widespread dissatisfaction with these drugs is due to two main reasons: (i) the incidence of adverse effects, which result in more than 7 million hospitalizations each year, and (ii) the inability to reverse the course of the disease

Method used

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  • Useful properties of a bee venom protein and gene encoding same
  • Useful properties of a bee venom protein and gene encoding same
  • Useful properties of a bee venom protein and gene encoding same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0182] (Clone PX3.101 cDNA and deduced protein sequence)

[0183] A. Method

[0184] Based on the amino acid sequence (NEIFSR--SEQ ID NO:9) obtained by protein sequencing, degenerate oligonucleotide primers (5'ATGGATCCAAYGARATHTTYWSNAG 3'-SEQ IDNO:8) were designed (Y=C or T; R= A or G; H=A or C or T; W=A or T; N=A or C or G or T). All PX3.101 primers and oligo(dT) (5'TTGCGGCCGCTTTTTTTTTTTTTTTTTT3' - SEQ ID NO: 10) were synthesized and purified by Genemed Synthetics.

[0185] Total RNA from bee venom was prepared as previously described (Chomczynski et al. (1995) Annals Biochemistry 225:163). Venom glands were collected from honeybees by Apitronic Services (Richmond, British Columbia, Canada) and stored at -80°C. One hundred venom glands were homogenized in STAT-60 solution (TEL-TEST "B" Company, TX) using a glass-Teflon homogenizer. Total RNA was extracted and isolated using an RNA isolation kit from TEL-TEST "B" company.

[0186] First-strand cDNA was synthesiz...

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Abstract

The invention provides a novel protein, PX3.101, which can be isolated from honey bee venom, antibodies against the polypeptide and nucleic acids encoding PX3.101 and fragments thereof. The invention also provides pharmaceutical compositions based upon PX3.101 polypeptide and methods for using same in the treatment of various diseases, including various inflammatory diseases such as rheumatoid arthritis.

Description

[0001] Cross-reference to related applications [0002] This application claims the benefit of US Provisional Application 60 / 100172, filed September 14, 1998, which is hereby incorporated by reference in its entirety. field of invention [0003] The present invention relates to the field of cloning and expression of proteins with therapeutic functions in the treatment of various diseases, especially inflammatory conditions such as rheumatoid arthritis. More specifically, the present invention relates to a novel protein called PX3.101 purified from honeybee venom and the gene encoding the protein. Background of the invention [0004] The immune system plays an extremely important beneficial role in fighting infection. However, in some cases, an inappropriate immune response can lead to many crippling diseases. Autoimmune or immune system mediated diseases can be either B-cell mediated (ie, antibody mediated) or T-cell m...

Claims

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Application Information

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IPC IPC(8): A61K38/00A61K39/00C07K14/435
CPCA61K38/00C07K14/43572A61K39/00A61P1/00A61P17/06A61P25/00A61P25/28A61P35/04A61P37/00
Inventor 崔相民吕越峰
Owner PAN PACIFIC PHARMA
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