Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Vitrification freezing, simple defreezing and direct implanting method for embryo

A vitrification and thawing technology, applied in the field of embryonic biology, can solve the problems of cumbersome thawing process, restrictions on the popularization and application of vitrification method, easy loss of embryos, etc., and achieve the effect of strong operability, increased length and good effect

Inactive Publication Date: 2007-04-04
CHINA AGRI UNIV
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The thawing process is not only very cumbersome, but also easy to lose embryos, requiring skilled technicians
These conditions have restricted the popularization and application of vitrification freezing method in production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vitrification freezing, simple defreezing and direct implanting method for embryo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] In the tube packing method as shown in Figure 1, 1 is cotton plug and sealing; 2 is sealing liquid (its composition is the same as 6); 3 is air segment; 4 is the freezing liquid that contains embryo; 5 is freezing liquid; 6 For thawing liquid.

[0020] The inventive point of the vitrified embryo freezing method of the present invention is that the configuration of the freezing liquid and the thawing liquid in the thin tube is different from other methods. It can be seen from Figure 1 that, compared with the "5-segment method" of the existing vitrified embryo freezing technology, this tube arrangement reduces the number of air segments and shortens the length of the freezing liquid segment, which can ensure that more embryos are used. Thawing solution to dilute the high-concentration freezing solution to complete the process of removing the antifreeze protection agent inside the embryo. Because the freezing liquid section is very short, if the embryos are loaded, the em...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The present invention discloses embryo vitrifying freezing, defreezing and directly transplanting method, and belongs to the field of embryo biotechnology. The technological scheme includes setting freezing liquid and defreezing liquid in different sections of fine tube separated with air, setting embryo in one section of the freezing liquid, sealing ends of the fine tube, and throwing the fine tube into liquid nitrogen; and features that there are two sections of freezing liquid and two sections of defreezing liquid with volume ratio between the freezing liquid and defreezing liquid of 1 to 5, or antifreeze protecting agent concentration in the mixed liquid of 2.0 mol / L. The simple defreezing and transplanting process includes taking out the fine tube from liquid nitrogen, air bathing for 4-6 sec, water bathing at 20-25 deg.c, shaking for homogeneous mixing of freezing liquid and transplanting. The present invention realizes the simple tube detoxication of embryo and direct transplanting.

Description

technical field [0001] The invention relates to a vitrified embryo freezing, thawing and direct transplantation method, in particular to a mammalian embryo freezing, thawing and transplantation method, and belongs to the field of embryonic biotechnology. technical background [0002] In 1972, Whittingham first used dimethyl sulfoxide (Dimethylsulfoxide: DMSO) as the main anti-cryoprotectant to cryopreserve 2-8-cell embryos of mice successfully, and transplanted them to give birth to offspring. The concentration of the freezing solution they used was 1.5Mol / L, and a programmed cooling freezer was used. After freezing and thawing, the embryo survival rate was 50% to 70%, the embryo pregnancy rate was 65%, and the litter rate was 48%. Since the invention of the programmed freezing method, researchers from various countries have repeatedly tested and screened various cryoprotectants, so that the survival rate of embryos after freezing and thawing can reach more than 90%, while ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/06C12N5/08
Inventor 朱士恩杨中强侯云鹏
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com