Multiplex PCR rapid identification method for salmonella and listeria monocytogenes

Abstract

The invention discloses a kind of appraisal bacterium, specially appraises the monk fungus (Salmonella), produces the uninuclear cell Liszt fungus (Listeria monocytogenes) the examination method. First (YAU_DSL1) separately uses the monk fungus and the backwoods coli the thermal crack solution to withdraw respective DNA template, produces the uninuclear cell Liszt fungus to use the enzyme solution to withdraw the DNA template, then carries on disposable PCR to expand increases the specific many genes, finally passes through the electricity to swim the appraisal. The invention through sample DNA, disposable at the same time to the monk fungus histidine transportation operon gene which possibly has, produces the uninuclear cell hemolysin gene to expand increases (YZU_DSL1) the material particle carries on with the certain existence backwoods coli expands increases, forms the massive genes duplication fragment, quite is convenient, can appraise whether for the monk fungus, produces the uninuclear cell Liszt fungus, and makes the judgement to the negative result reacting system accuracy. The invention more classical method bacterium separation, the appraisal and the blood serum school grades testing method fast, is accurate.

Description

technical field [0001] The invention discloses a detection method for identifying bacteria, in particular for identifying Salmonella and Listeria monocytogenes. Background technique [0002] Salmonella and Listeria monocytogenes are important zoonotic pathogens, and are the two most important foodborne pathogenic bacteria listed by the World Health Organization (WHO). Salmonella can not only cause animal diseases, but also cause typhoid fever, paratyphoid fever, sepsis, gastroenteritis and food poisoning in humans. In food poisoning around the world, the poisoning cases caused by Salmonella account for the first or second place; Listeria is A food-borne pathogen that can cause diseases such as abortion, sepsis and meningitis in animals and humans. Newborns, the elderly, pregnant women and immunocompromised patients are high-risk groups for Listeria infection. In countries all over the world, including the United States and other developed countries, food contamination by Sa...

AI Technical Summary

Problems solved by technology

Traditional methods of isolation, culture and serology are commonly used in the detection of Salmonella and Listeria monocytogenes. Different testing procedures and methods are used, and they are reported separately. It takes at least 5 to 7 days for the confirmed test results. The testing methods are cumbersome, time-consuming and laborious. Low specificity defects, especially my country has joined the World Trade Organization (WTO), and the volume of international trade is increasing day by day