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Odd number agar oligosaccharide monomer and its preparation method

A technology of agar oligosaccharides and monomers, applied in the direction of oligosaccharides, etc., can solve the problems of low solubility, large molecular weight, high viscosity, etc.

Inactive Publication Date: 2006-05-17
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its application is greatly limited due to its large molecular weight, high viscosity, and low solubility.
At present, people can study the chemical structure of agar by acid degradation, and some people have prepared agar oligomers and oligosaccharide mixtures by acid hydrolysis, but no odd-numbered agar oligosaccharide monomers have been prepared by acid hydrolysis.

Method used

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  • Odd number agar oligosaccharide monomer and its preparation method
  • Odd number agar oligosaccharide monomer and its preparation method
  • Odd number agar oligosaccharide monomer and its preparation method

Examples

Experimental program
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Embodiment 1

[0010] Take 10g of agarose, add 1000ml of distilled water and heat it to dissolve, add 5ml of 2mol / L sulfuric acid aqueous solution, stir and hydrolyze in a water bath at 80°C for 240min, after the reaction is completed, cool the reactant to room temperature with a cold water bath, add barium carbonate to neutralize To neutral, filter with suction, concentrate the filtrate to 100ml under reduced pressure at 40°C, add 4 times the amount of ethanol to cause precipitation, centrifuge, concentrate the supernatant under reduced pressure at 40°C, and freeze-dry to obtain an oligosaccharide mixture. Take 2g of the oligosaccharide mixture, add 1ml of water to dissolve it, inject it into a Superdex30 chromatographic column, and elute with distilled water at room temperature, combine the eluents from each sugar peak, concentrate under reduced pressure, and freeze-dry to obtain agarose and agarose pentaose , Qiongqiose, Qiongjiutang.

Embodiment 2

[0012] Take 100g of agarose, add 5000ml of distilled water and heat to dissolve, add 15ml of 3mol / L hydrochloric acid solution, stir and hydrolyze in a water bath at 70°C for 7h, after the reaction is completed, cool the reactant to room temperature in a cold water bath, and add barium carbonate to neutralize To neutral, filter with suction, concentrate the filtrate to 200ml under reduced pressure at 40°C, add 4 times the amount of methanol to cause precipitation, centrifuge, concentrate the supernatant under reduced pressure at 40°C, and freeze-dry to obtain the oligosaccharide mixture. Take 10g of the oligosaccharide mixture, add 1ml of water to dissolve it, inject it into a Bio-Gel-P6 chromatographic column, and elute with distilled water at room temperature, combine the eluents of each sugar peak, concentrate under reduced pressure, and freeze-dry to obtain agarose , Qiongpentose, Qiongqiose, Qiongjiutang.

[0013] The agar or agarose described in the present invention is ...

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Abstract

An odd agaropectin oligose monomer, such as agartriose, agarpentose, agarheptose, or agarnonose, is prepared through adding water to agaropectin or agaropectinose, adding acid, thermal reacting at 50-90 deg.C for 15-480 min, centrifugal separation, evaporation concentrating, adding organic solvent, centrifugal separation, concentrating supernatant, chromatographic column separation, and freeze drying.

Description

technical field [0001] The invention relates to an odd-numbered agar oligosaccharide monomer and a preparation method thereof. Background technique [0002] Agarose is a neutral sugar composed of agarose skeleton, which transitions from a series of agarose containing low charge and pyruvic acid to a series of continuous agarose derivative molecules with high charge and acidic substituents. Mixture composition. It has been widely used in food industry, medicine, hygiene and scientific research for a long time (Ji Minghou, Seaweed Chemistry, Science Press, Beijing, 1997). However, its application is greatly limited due to its large molecular weight, high viscosity and low solubility. At present, people can study the chemical structure of agar by acid degradation, and some people have prepared agar oligomers and oligosaccharide mixtures by acid hydrolysis, but no odd-numbered agar oligosaccharide monomers have been prepared by acid hydrolysis. Contents of the invention [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06C08B37/00
Inventor 毛文君管华诗
Owner OCEAN UNIV OF CHINA
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