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Prepn of biocompatible four component microcapsule

A biocompatible, four-component technology, which is applied in the field of four-component microcapsule preparation, can solve the problems of inability to culture microcapsule cells, impure components of composition materials, and no report on the preparation process, etc., so as to achieve easy application Effects with promotion, cheap raw materials, and good biocompatibility

Inactive Publication Date: 2005-05-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At the beginning of this century, a multi-component microcapsule system appeared, consisting of sodium alginate (SA) / cellulose sodium sulfate (CS)-calcium chloride (CaCl 2 ) / polymethylenebiguanidine · hydrogen chloride (PMCG) four-component capsules, but the literature does not report its specific preparation process, and because of the impurity of the constituent substances or improper composition of the components, the obtained microcapsules It is toxic to microorganisms, etc., so it cannot be used as a biomicrocapsule for cell culture

Method used

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  • Prepn of biocompatible four component microcapsule
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  • Prepn of biocompatible four component microcapsule

Examples

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preparation example Construction

[0026] The materials used for the preparation of novel biological microcapsules of the present invention are as follows:

[0027] 1) Sodium cellulose sulfate (CS), prepared by the Institute of Bioengineering, Zhejiang University.

[0028] 2) Sodium alginate (SA), purchased from Shanghai Institute of Biochemistry.

[0029] 3) Polymethylenebiguanidine·hydrogen chloride (PMCG), which was purchased from Scientific Polymer Products, USA, and other reagents such as calcium chloride were commercially available analytical reagents.

[0030] Different examples are given below to illustrate the present invention:

Embodiment 1

[0032]1) At room temperature, fully mix absolute ethanol and PMCG aqueous solution at a ratio of 2:1, let stand for 30 minutes, centrifuge the mixture at 6000rpm for 15min, collect the precipitate, wash it with absolute ethanol for 3 times, and then wash the precipitate The product was freeze-dried to remove residual ethanol and other small molecular impurities. Finally, the precipitate was dried in a vacuum oven at 50° C. for 48 hours and set aside;

[0033] 2) Fully mix 2% sodium alginate (SA) and 3.5% cellulose sodium sulfate (CS), the ratio of the two is 1.5, this mixture is the polyanion solution of this system, pass the polyanion solution through the syringe Drop in 3% calcium chloride solution and solidify, solidify time is 15 minutes, obtain solid pre-capsule, wash pre-capsule with deionized water, then transfer it to 2% polymethylene bismuth React in guanidine·hydrogen chloride (PMCG) solution, and the reaction time is 15 minutes, and the obtained capsule gets final ...

Embodiment 2

[0036] 1) Fully mix absolute ethanol and PMCG aqueous solution at a ratio of 0.5:1, let stand for 25 minutes, centrifuge the mixture at 4000 rpm for 5 minutes, collect the precipitate, wash it twice with the corresponding organic solvent, and then freeze the precipitate Dry to remove residual organic solvents and other small molecular impurities, and finally dry the precipitate in a vacuum oven at 37°C for 24 hours to obtain polymethylenebiguanidine·hydrogen chloride (PMCG) from which small molecular impurities are removed;

[0037] 2) Fully mix 0.5% sodium alginate (SA) and 0.5% cellulose sodium sulfate (CS), the ratio of the two is 0.2, this mixture is the polyanion solution of this system, pass the polyanion solution through the syringe Drop into 0.5% calcium chloride solution to solidify, and the solidification time is 5 minutes to obtain a solid pre-capsule, wash the solid pre-capsule with deionized water, and then transfer it to 1% polymethylene bismuth to remove small mo...

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Abstract

The invention discloses a preparation method of four-component microcapsules with biocompatibility. The multi-component system is composed of sodium alginate (SA), sodium cellulose sulfate (CS), calcium chloride and polymethylene biguanidine hydrogen chloride (PMCG). The mixture of SA and CS is dropped into calcium chloride Solid pre-capsules are formed in the solution, and then transferred into PMCG for reaction to obtain microcapsules. The commercially available raw material PMCG is a high-viscosity aqueous solution containing 35% PMCG, which contains small molecular impurities that are toxic to microorganisms. After ethanol precipitation, centrifugation, vacuum drying and freeze-drying, the harmful substances can be completely removed. . The capsules prepared by the present invention can not only independently adjust the parameters of the capsules, such as: capsule size, film thickness, mechanical strength and permeability, and the characteristics of the capsules can be adjusted and optimized according to different requirements of practical applications; Capacitive, is a promising and well-performing immobilization system for cell culture.

Description

technical field [0001] The invention relates to a preparation method of four-component microcapsules with biocompatibility. Thereby, the cultivation of microbial cells can be carried out. Background technique [0002] Biological microcapsule technology is a high-tech driven and developed for organ transplantation. The use of cell transplants to treat various human diseases, such as hormonal or protein deficiencies, is limited because the transplanted cells are quickly destroyed by the recipient's immune system. To overcome this obstacle, cells that secrete hormones or proteins must be wrapped in a semi-permeable membrane to protect them from the recipient's immune system, but at the same time allow small molecules that are important for cell function or survival (such as oxygen and nitrogen, etc.) ) entry and exit of desired cell products. The semipermeable membrane of the biomicrocapsule has the function of immune isolation. Therefore, the p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02C12N1/00C12N11/02
Inventor 姚善泾张立央关怡新
Owner ZHEJIANG UNIV
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