Construction method for skin tissue engineering rack containing epidermal growth factor

Abstract

The invention relates to a construction method of a skin tissue engineering scaffold containing epidermal growth factor, which is characterized in that it uses fetal cowhide or bovine Achilles tendon as a base material, undergoes dehydration and degreasing treatment, and performs an enzyme treatment to reduce the co-production between tissue fibers. valence bond, and then through folding, carry out secondary treatment, remove the antigenic determinant, then dissolve with acetic acid to obtain non-antigenic collagen solution, then make collagen sponge membrane, soak and absorb with glycerol, after drying, heparin acetic acid aqueous solution The mixture with the aqueous solution of epidermal growth factor and glycerol is mixed again to wet the collagen sponge membrane, and then freeze-dried to construct a scaffold for skin tissue engineering. The scaffold has the advantages and effects of no antigenicity, high strength, good flexibility, convenient surgical implantation, no toxicity to the wound surface, effective induction of autologous cell growth at the wound surface, and promotion of wound healing.

Description

technical field [0001] The invention belongs to the class of dressings for medical devices, specifically a method for constructing a skin tissue engineering scaffold by adopting a tissue engineering method and taking fetal cowhide and bovine Achilles tendon collagen as scaffolds. Background technique [0002] With the emergence and development of tissue engineering science, research on skin wound repair dressings has shifted from pure wound dressing research to active artificial skin research. Active artificial skin constructed by tissue engineering has become the best choice for skin wound repair. [0003] The research on skin tissue engineering scaffold is one of the focuses of tissue engineering research. It has a history of nearly 20 years. In-depth studies have shown that although polyesters have good biological properties, the degradation products will increase the acidity of the surrounding tissues, which is limited in use; natural biomaterials mainly composed of coll...

AI Technical Summary

Problems solved by technology

[0005] The collagen sponge membrane formed by freeze-drying the collagen solution has poor elasticity, is easily broken, and has poor stability, so it needs to be cross-linked by adding a cross-linking agent or undergoing special treatment.

[0006] Among the existing technologies that use collagen as raw material to make collagen sponge membranes as wound dressings, some use glutaraldehyde as a crosslinking agent to improve the strength, toughness and stability of collagen sponge membranes, but reports have confirmed that Dialdehyde has obvious cytotoxicity. When cells are cultured with a sponge membrane containing glutaraldehyde, there is little cell ingrowth, and as the collagen sponge membrane gradually degrades in the body, glutaraldehyde monomers will also be released into body fluids Among them, it is harmful to the human body, so glutaraldehyde is not an ideal cross-linking agent; some use chondroitin sulfate as a cross-linking agent to improve the strength of the collagen sponge membrane and the tolerance to enzymes, but chondroitin sulfate and collagen Molecules can only be bonded with weak bonds, which has the disadvantages of promoting scar formation and reducing collagen coagulation ability, and is also not an ideal cross-linking agent