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Bacteria-enzyme synergistic fermented high-protein eggshell membrane powder as well as preparation method and application thereof

A technology of co-fermentation and eggshell membrane, applied in application, food processing, animal husbandry, etc., can solve the problems of high cost of feed protein raw materials, shortage of resources, etc., to improve production performance and blood indicators, good economic benefits, and expand feed The effect of resources

Pending Publication Date: 2022-08-09
WUHAN SUNHY BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the invention is to overcome the problems of high cost of feed protein raw materials and shortage of resources in the prior art

Method used

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  • Bacteria-enzyme synergistic fermented high-protein eggshell membrane powder as well as preparation method and application thereof
  • Bacteria-enzyme synergistic fermented high-protein eggshell membrane powder as well as preparation method and application thereof
  • Bacteria-enzyme synergistic fermented high-protein eggshell membrane powder as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The present embodiment provides a high-protein eggshell film powder and a preparation method thereof fermented by bacteria and enzymes, comprising the following steps:

[0023] (1) The eggshell membrane is washed and dried, pulverized with a universal pulverizer, and passed through a 120-mesh sieve;

[0024] (2) Accurately weigh 4g of the sieved eggshell membrane, add it to 18ml of MRS broth medium, seal it with cotton plugs and kraft paper, and sterilize at 121°C for 30min;

[0025] (3) Preparation of compound enzyme preparation: Weigh untreated compound enzyme, wherein the enzyme activity of aminopeptidase is 2×10 6 U / g, the enzyme activity of bacterial alkaline protease is 2×10 6 U / g, the enzyme activity of Aspergillus oryzae neutral protease is 1×10 6 U / g, the untreated compound enzyme was added to the nutrient broth medium with the addition amount of 250g / t to obtain the compound enzyme preparation, and after the preparation was completed, it was filtered on the ...

Embodiment 2

[0031] In this example, the same method as in Example 1 was used to prepare a large amount of high-protein eggshell membrane powder co-fermented with bacteria and enzymes, and it was used for feeding broilers to study the feeding effect of the high-protein eggshell membrane powder.

[0032] Before raising chickens, do a good job of cleaning and disinfection of equipment and chicken houses. Clean the chicken houses thoroughly. After drying, use potassium permanganate and formaldehyde to fumigate. A total of 144 AA broilers with similar body weight at 1-day age and in good health were selected and randomly divided into control group 1, control group 2 and experimental group, with 3 replicates in each group, and 16 broilers in each replicate (half male and female). Keep the room well ventilated, the room temperature is maintained at 22-25 ℃, the broilers are raised on the ground level, free to eat and drink, the immunization program and the deworming program are carried out synchr...

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Abstract

The invention belongs to the technical field of feed additives, and particularly provides bacterium-enzyme synergistic fermented high-protein eggshell membrane powder which comprises the following components in percentage by mass: 30-35% of a compound enzyme preparation, 5-8% of a compound bacterium agent and 57-65% of an eggshell membrane, wherein the compound enzyme preparation comprises aminopeptidase, bacterial alkaline protease and aspergillus oryzae neutral protease; the complex microbial inoculants comprise lactobacillus plantarum, bifidobacterium breve, bacillus subtilis, bacillus amyloliquefaciens and bacillus licheniformis. The complex microbial inoculant and the complex enzyme preparation synergistically act on the eggshell membrane, so that on one hand, the use effect of the eggshell membrane is improved, and the amount of eggshell membrane garbage is reduced; on the other hand, the feed cost increase caused by feed protein raw material price rise is reduced. Besides, when the high-protein eggshell membrane powder provided by the invention is applied to broiler feed, the production performance and blood indexes of broilers can be improved, the death and culling rate can be reduced, and good economic benefits are achieved.

Description

technical field [0001] The invention belongs to the technical field of feed additives, in particular to a high-protein eggshell membrane powder fermented by bacteria and enzymes, and a preparation method and application thereof. Background technique [0002] Eggshell membrane is a thin film in the inner layer of eggshell, and its main component is fibrous protein in network structure. Eggshell Membrane Powder stabilizes and enhances joint health, acts as a source of dietary calcium, has anti-inflammatory and wound healing properties, and has positive effects in the treatment of inflammatory bowel disease. Eggshell membranes are often used in cosmetics, personal care industries and food industries, but in terms of overall output, eggshell membranes that are fully used are still a minority, and most of them are treated as waste. Only in European countries, the annual amount of eggshell waste is It can reach 1 million tons, and the eggshell membrane is not fully utilized, resu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/26A23K10/12A23K10/14A23K50/75
CPCA23K10/26A23K10/12A23K10/14A23K50/75A23V2400/519A23V2400/169Y02P60/87
Inventor 付大波王铕张成杰张云非张小龙王子蔚熊晓燕周樱詹志春
Owner WUHAN SUNHY BIOLOGICAL
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