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Quercus variabilis QsSRO1 gene and application of encoding protein of Quercus variabilis QsSRO1 gene in plant stress resistance

A technology of plant stress resistance and cork oak, which is applied in the field of genetic engineering, can solve the problems of slow breeding process, complicated process, and separation of traits, and achieve the effects of reducing damage, strong tolerance, and improving stress resistance

Active Publication Date: 2022-08-02
青岛斯坦德衡立环境技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hybridization process does not produce new genes, and the hybrid offspring will have traits separated, and the breeding process is slow and complicated

Method used

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  • Quercus variabilis QsSRO1 gene and application of encoding protein of Quercus variabilis QsSRO1 gene in plant stress resistance
  • Quercus variabilis QsSRO1 gene and application of encoding protein of Quercus variabilis QsSRO1 gene in plant stress resistance
  • Quercus variabilis QsSRO1 gene and application of encoding protein of Quercus variabilis QsSRO1 gene in plant stress resistance

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Embodiment 1

[0021] Example 1: Quercus cork QsSRO1 gene and its obtaining method

[0022] 1. How to obtain QsSRO1 of Quercus cork

[0023] Extract the RNA of Quercus cork and use TaKaRa PrimeScriptTM as the RNA template RT The kit is reverse transcribed into cDNA by the reverse transcription kit method. Then, the cDNA was used as a reaction template for amplifying the target gene sequence, and 5'-ATGGCGAATCTACCCCAATCGC-3' and 5'-TCAGTTTCCGGGCTCTGGTTTC-3' were used as primers for PCR amplification to obtain the CDS sequence of the QsSRO1 gene.

[0024] 2. Analysis of tissue expression of QsSRO1 gene by real-time quantitative PCR

[0025] The RNA was extracted from the roots, stems and flowers of Quercus cork, and cDNA was synthesized by reverse transcription. Then, the cDNAs of the above tissues were used as templates, and 5'-AGACGGGACGAGGGTTGT-3' and 5'-TGGATGATGTGGGCTTGG-3' were used as primers for PCR amplification. To detect the expression of QsSRO1 gene in different tissues of Querc...

Embodiment 2

[0028] Example 2: Application of QsSRO1 gene in improving plant stress resistance

[0029] 1. Construction of Arabidopsis transfected with QsSRO1

[0030] To obtain the QsSRO1 gene of Quercus cork, the following primer pairs were prepared:

[0031] Primer 1: 5'-ATGGCGAATCTACCCCAATCGC-3';

[0032] Primer 2: 5'-TCAGTTTCCGGGCTCTGGTTTC-3'.

[0033] Using the cDNA of Quercus cork as a template, PCR amplification was carried out using the above primers 1 and 2 to obtain a PCR product with a size of 1755 bp, which was sequenced. The obtained PCR product is the QsSRO1 gene of Quercus cork, and the nucleotide sequence shown by the sequencing result is shown in SEQ ID NO.1, which can encode the QsSRO1 protein whose amino acid sequence is shown in SEQ ID NO.2. Bioinformatics analysis showed that the protein encoded by the QsSRO1 gene contained 585 amino acid residues, with a relative molecular mass of 66.04kDa and a theoretical isoelectric point of 5.85. The QsSRO1 protein contains a...

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Abstract

The invention belongs to the technical field of gene engineering, and particularly relates to an application of a Quercus variabilis QsSRO1 gene and an encoding protein thereof in plant stress resistance. The nucleotide sequence of the Quercus variabilis QsSRO1 gene is as shown in SEQ ID No.1, the amino acid sequence of Quercus variabilis QsSRO1 protein coded by the Quercus variabilis QsSRO1 gene is as shown in SEQ ID No.2, and the Quercus variabilis QsSRO1 gene is a cell nucleus protein. A recombinant expression vector containing the Quercus variabilis QsSRO1 gene is constructed, Arabidopsis thaliana is transformed by Agrobacterium tumefaciens, and QsSRO1 transgenic Arabidopsis thaliana plant seeds obtained after screening and cultivation have good performance in salt resistance tests. The invention proves that overexpression of the Quercus variabilis QsSRO1 gene can improve the salt resistance of plants, and a theoretical and production practice foundation is laid for cultivating new varieties of salt-resistant and drought-resistant transgenic crops.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to the application of the Quercus cork QsSRO1 gene and its encoded protein in plant stress resistance. Background technique [0002] The cork oak (Quercus suber) is an important broad-leaved evergreen tree species with slow growth and extremely long lifespan. Cork oak is a major source of industrial cork, a material of high commercial value. [0003] Climate change is one of the major challenges and global issues facing the 21st century. In recent decades, rising global temperatures have caused a series of more intense, frequent and longer-lasting climate extremes, especially severe drought and soil salinization. Several global changes, including soil salinization, have been reported to cause severe declines in cork oak populations. Although tree species can adapt to new environmental conditions, little is known about the processes involved. Conventional cross-breedi...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N1/21A01H5/10A01H5/00A01H6/20C12R1/01
CPCC07K14/415C12N15/8273C12N15/8205
Inventor 袁本起宋文涛杨敬杰黄婷婷邓传光于媛媛
Owner 青岛斯坦德衡立环境技术研究院有限公司
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