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Polypeptide and application thereof in promoting proliferation of human natural killer cells and preparing human natural killer cell culture medium

A natural killer cell and culture medium technology, which is applied to animal cells, vertebrate cells, blood/immune system cells, etc., can solve problems such as limiting large-scale research experiments, and achieve the promotion of human natural killer cell proliferation and human natural killer cells. The effect of killer cell proliferation in vitro

Pending Publication Date: 2022-08-02
陈飞
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  • Claims
  • Application Information

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Problems solved by technology

Due to the low number of natural killer cells in the human body, large-scale research experiments are limited

Method used

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  • Polypeptide and application thereof in promoting proliferation of human natural killer cells and preparing human natural killer cell culture medium
  • Polypeptide and application thereof in promoting proliferation of human natural killer cells and preparing human natural killer cell culture medium
  • Polypeptide and application thereof in promoting proliferation of human natural killer cells and preparing human natural killer cell culture medium

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Embodiment Construction

[0015] The following examples are only used to specifically introduce the essential content of the present invention, but do not limit the protection scope of the present invention.

[0016] 1. Experimental materials

[0017] Polypeptide J whose sequence is RNDWTMDEQ (Sequence NO. 1) was synthesized by Nanjing Peptide Valley Biotechnology Co., Ltd. by conventional solid-phase synthesis method. The molecular formula of polypeptide J is C 48 H 71 N 15 O 19 S, molecular weight is 1194.23, molecular ion peak [M+H] detected by mass spectrometry + 1195.2. HPLC purity 95.327%, see figure 1 .

[0018] NK cell culture medium, Saide Biopharmaceutical Co., Ltd. (the main components are RPMI 1640, IL-15, IL-2, SCF, only used for in vitro expansion and culture of NK cells isolated from human lymphocytes, without Cell selection, induction, differentiation function); RPMI 1640 medium and fetal bovine serum, GIBCO company.

[0019] MTT reagent and monoclonal antibody as well as Weste...

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Abstract

The invention discloses a polypeptide and application thereof in promoting proliferation of human natural killer cells and preparing a human natural killer cell culture medium. The natural killer cell is an anti-tumor and anti-virus innate immune cell, plays an important role in natural immunity, also plays an important role in the aspects of early-stage anti-tumor and immune surveillance, and can directly kill tumor cells without antigen specific recognition. As the number of natural killer cells in a human body is small, large-scale research experiments are limited. The polypeptide has the effect of promoting proliferation of the human natural killer cells and can be used for developing and preparing a culture medium for promoting in-vitro proliferation of the human natural killer cells.

Description

technical field [0001] The invention belongs to the field of biochemistry and relates to polypeptide and natural killer cell culture, in particular to a polypeptide and its application in promoting human natural killer cell proliferation and preparing human natural killer cell culture medium. Background technique [0002] In the early 1970s, researchers discovered spontaneous cytotoxicity in lymphocytes and found a new lymphocyte subset, which was named natural killer cells (NK). Natural killer cells are innate immune cells that fight tumors and viruses. They play an important role in innate immunity, and also play an important role in early anti-tumor and immune surveillance. They can directly kill tumor cells without antigen-specific recognition. Large-scale research experiments are limited due to the low number of natural killer cells in the human body. It has become a hot issue to study its cell biological characteristics and expand in vitro to obtain a large number of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12N5/0783
CPCC07K7/06C12N5/0646C12N2501/998
Inventor 陈飞
Owner 陈飞
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