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Polypeptide and preparation method thereof, and organic compound trace element and preparation method thereof

A technology of compounding trace elements and trace elements, which is applied in the field of feed processing, can solve problems such as unfavorable enzymatic hydrolysis and low soluble protein content, and achieve the effects of improving functional properties, increasing the number of action sites, and improving solubility

Pending Publication Date: 2022-07-22
CHENGDU SHUXING FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soybean meal is rich in protein resources, but it has not been popularized and applied because it contains anti-nutritional factors, low soluble protein content, and is not conducive to enzymatic hydrolysis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] First, the present invention provides a method for preparing a polypeptide for organic composite trace elements, comprising the following steps:

[0023] S1 soybean meal powder is dissolved in water to form soybean meal liquid, which is treated by jet cooking to obtain the enzymatic hydrolyzate;

[0024] In S2, the product to be hydrolyzed is subjected to enzymatic hydrolysis, and after adding phosphate, it is mixed with polyvinylpyrrolidone, reacted at a constant temperature, and concentrated to obtain a polypeptide.

[0025] In the present invention, the mass ratio of soybean meal powder, phosphate and polyvinylpyrrolidone is 100:0.1-1.5:5-25.

[0026] In the present invention, in S1, the process of jet cooking is as follows: the temperature is 110-140°C, and the time is 10-120s.

[0027] In the present invention, in S1, the solid-liquid mass ratio of soybean meal powder and water is 1:5-30.

[0028] In the present invention, in S2, the enzymes used are alkaline pro...

Embodiment 1

[0046] An organic complex trace element, including polypeptide and trace element zinc.

[0047] The preparation method of the polypeptide is,

[0048] S1 soybean meal powder is dissolved in water, and the mass ratio of soybean meal powder to water is 1:20 to form soybean meal liquid, which is subjected to jet cooking treatment at a temperature of 120 °C and a time of 85 s to obtain the enzymatic hydrolysis product;

[0049] S2 treats the product to be hydrolyzed by enzymolysis, the amount of enzyme is 400U / g, and the time of enzymolysis is 6h. The enzymes included alkaline protease and flavor protease (3:1 mass ratio). After the enzymolysis treatment, the pH value is adjusted to 4-5, and after centrifugal precipitation, the proteolysis product is obtained. The proteolysis product is added with water to form a saturated solution, and then tripolyphosphate is added for blending, and then blended with polyvinylpyrrolidone. The reaction temperature is 70°C, and the reaction time...

Embodiment 2

[0057] The difference between this example and Example 1 is that the parameters of the jet cooking treatment are that the temperature is 135° C. and the time is 50 s.

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Abstract

The invention relates to the technical field of feed processing, and aims to solve the problem of low polypeptide chelation degree mentioned in the prior art. The invention provides polypeptide and a preparation method thereof as well as organic compound trace elements and a preparation method thereof, and the preparation method of the polypeptide comprises the following steps: S1, adding water into soybean meal powder for dissolving to form soybean meal liquid, and carrying out jet cooking treatment to obtain a to-be-enzymolyzed substance; and S2, carrying out enzymolysis treatment on the to-be-enzymolyzed product, adding phosphate, blending with polyvinylpyrrolidone, carrying out constant-temperature reaction, and concentrating to obtain the polypeptide. Through jet cooking and the action of phosphate, protein aggregation is avoided, and the solubility is improved. On the basis that the content of soluble protein in the soybean meal powder is increased, acting sites of the soybean meal powder and protease in enzymatic hydrolysate are increased, then multi-activity polypeptide can be obtained, and the chelation degree of the soybean meal powder is improved.

Description

technical field [0001] The present invention relates to the technical field of feed processing, in particular to polypeptides and preparation methods, organic composite trace elements and preparation methods. Background technique [0002] Zinc is an essential trace element for animals. It is related to the activity of nearly 300 enzymes in the body. Some enzymes use zinc as their structure, and some use zinc as their cofactor. Zinc regulates and controls the structure and function of these enzymes. Thus affecting many metabolic functions of the matrix. [0003] Traditionally, the zinc supplements used in pig feed are mainly inorganic zinc. The free metal ions released by inorganic zinc during the digestion process are easily combined with some anti-nutritional factors in the intestine to form insoluble salts or precipitate out of the body, thereby reducing metal ions. absorption of ions. Peptides are a class of active substances in living organisms and participate in many ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/107A23K20/147A23K20/20C08G81/00
CPCC12P21/06C07K1/1077A23K20/147A23K20/20C08G81/00
Inventor 王韶辉李芳
Owner CHENGDU SHUXING FEED
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