Stacked fixed bed bioreactor and method of use thereof

Abstract

A modular and stacked cell culture system includes individual cell culture subunits having an internal chamber for containing a cell culture substrate in a cell culture space, a fluid inlet for supplying fluid to the cell culture space, and a fluid outlet for removing fluid from the chamber. The chamber is arranged for fluid to flow in from the fluid inlet, through the cell culture space, and then out through the fluid outlet. The subunit further includes an alignment feature on at least one of a top and a bottom of an individual cell culture subunit, where the alignment feature is aligned with an alignment feature of another individual cell culture subunit such that a plurality of individual cell culture subunits are stackable.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS [0002] This application claims the benefit of priority under 35 U.S.C § 120 to US Provisional Application Serial No. 62 / 941,308, filed November 27, 2019, the contents of which are hereby incorporated by reference in their entirety. technical field [0003] The present disclosure relates generally to the field of bioprocessing and, in particular, to modular and stacked packed bed bioreactors and methods of using the bioreactors for cell culture. Background technique [0004] In the bioprocessing industry, large-scale cell culture is performed for the production of hormones, enzymes, antibodies, vaccines, and cell therapy. Most cells used in bioprocessing are anchorage-dependent, meaning that cells need a surface to adhere to in order to grow and function. Traditionally, culturing of adherent cells has been performed on two-dimensional (2D) surfaces of adherent cells contained in one of a variety of container formats such as T-...

AI Technical Summary

Problems solved by technology

However, such a design greatly affects the reactor scale-up in bioprocessing therapeutic manufacturing

For example, while suspension st

Images