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Construction method of metabolism-related fatty liver disease non-human primate model

A technology for primates and fatty liver disease, which is applied in the field of construction of non-human primate models of metabolic-related fatty liver disease, and can solve problems such as differences in the risk factors for fatty liver disease

Pending Publication Date: 2022-07-12
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The Chinese patent discloses a non-alcoholic chronic steatohepatitis non-human primate model and its construction method and use, but the construction methods used include high-fat diet, CCL4 chemical induction and alcohol drinking, which is related to human metabolism. There are some differences in the predisposing factors of chronic liver disease

Method used

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  • Construction method of metabolism-related fatty liver disease non-human primate model
  • Construction method of metabolism-related fatty liver disease non-human primate model
  • Construction method of metabolism-related fatty liver disease non-human primate model

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Taking adult cynomolgus monkeys as an example, a non-human primate model of metabolic-related fatty liver disease with abnormal glucose and lipid metabolism and further characteristics of liver steatosis and fibrosis was constructed.

[0037]In this experiment, a high-fat, high-sugar, and high-cholesterol feed—F1 was prepared first, and its nutrients included 25wt% crude protein, 30wt% crude fat, 17wt% crude fiber, 3wt% fructose, 1wt% cholesterol, 4wt% calcium, 1wt% total Phosphorus, 0.7wt% vitamins, 5wt% minerals high-sugar, high-fat and high-cholesterol feed; the caloric composition of the high-fat, high-sugar and high cholesterol feed is: protein 1000Kcal / kg, fat 2700Kcal / kg, carbohydrates 960Kcal / kg, total The energy is 4660Kcal / kg; the fatty acid composition in the high-fat, high-sugar and high-cholesterol feed is: saturated fatty acid 51.58wt%, monounsaturated fatty acid 27.78wt% and polyunsaturated fatty acid 20.64wt%. Medium-chain fatty acids accounted for 6.8% ...

Embodiment 2

[0041] Taking adult cynomolgus monkeys as an example, a non-human primate model of metabolic-related fatty liver disease with abnormal glucose and lipid metabolism and further characteristics of liver steatosis and fibrosis was constructed.

[0042] In this experiment, a high-fat, high-sugar, and high-cholesterol feed—F2 was prepared first, and its nutrients included 22wt% crude protein, 35wt% crude fat, 14wt%w crude fiber, 15wt% fructose, 2wt% cholesterol, 2wt% calcium, 1wt% total Phosphorus, 0.2wt% vitamins, 0.5wt% minerals high-sugar, high-fat and high-cholesterol feed; the caloric composition of the high-fat, high-sugar and high cholesterol feed is: protein 880Kcal / kg, fat 3150Kcal / kg, carbohydrates 1160Kcal / kg, The total energy is 5190Kcal / kg; the fatty acid composition in the high-fat, high-sugar and high-cholesterol feed is: saturated fatty acid 57.58wt%, monounsaturated fatty acid 23.78wt% and polyunsaturated fatty acid 28.64wt%, the high-fat, high-sugar and high-choles...

Embodiment 3

[0046] Taking adult cynomolgus monkeys as an example, a non-human primate model of metabolic-related fatty liver disease with abnormal glucose and lipid metabolism and further characteristics of liver steatosis and fibrosis was constructed.

[0047] In this experiment, a high-fat, high-sugar, and high-cholesterol feed—F3 was prepared first. Its nutrients included 17wt% crude protein, 45wt% crude fat, 13wt% crude fiber, 15wt% fructose, 3wt% cholesterol, 4wt% calcium, 0.7wt% total Phosphorus, 0.48wt% vitamins, 0.6wt% minerals high-sugar, high-fat and high-cholesterol feed; the caloric composition of the high-fat, high-sugar and high cholesterol feed is: protein 680Kcal / kg, fat 4050Kcal / kg, carbohydrates 1120Kcal / kg, The total energy is 5850Kcal / kg; the fatty acid composition in the high-fat, high-sugar and high-cholesterol feed is: saturated fatty acid 51.53wt%, monounsaturated fatty acid 29.95wt% and polyunsaturated fatty acid 18.52wt%, the high-fat, high-sugar and high-choleste...

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Abstract

The invention discloses a construction method of a metabolism-related fatty liver disease non-human primate model, and belongs to the technical field of experimental animals. According to the construction method, non-human primate animals, namely cynomolgus monkeys, are used as model animals, and are fed with high-fat, high-sugar and high-cholesterol feed, so that the glycolipid metabolism disorder phenomenon that the weight of the animals is increased along with the feeding time, and the blood glucose, cholesterol and triglyceride levels are remarkably increased is avoided; therefore, liver fat accumulation and fibrosis lesion are caused. The metabolism-related fatty liver disease non-human primate animal model constructed by the invention can simulate the occurrence and development of the metabolism-related fatty liver in physiological and pathological processes caused by improper diet of human beings, and a preclinical evaluation tool is provided for developing the research and development of nutrients and medicines in related fields.

Description

technical field [0001] The invention belongs to the technical field of experimental animals, in particular to a method for constructing a non-human primate model of metabolic-related fatty liver disease. Background technique [0002] Metabolic associated fatty liver disease (MAFLD), formerly known as nonalcoholic fatty liver disease (NAFLD), has a global prevalence of up to 25%, seriously endangering human health and causing damage to society. huge economic burden. Unhealthy living habits such as sedentary and inactivity, excessive dietary calories, unreasonable dietary structure and other unhealthy eating habits are closely related to the increasing incidence of MAFLD. MAFLD has become the second largest chronic liver disease after viral hepatitis in my country, but its pathogenesis has not been fully elucidated, and there is a lack of effective treatment measures. [0003] Animal models play an important role in exploring the pathogenesis of diseases, evaluating diagnost...

Claims

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Application Information

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IPC IPC(8): A01K67/027A23K10/20A23K10/22A23K10/37A23K10/30A23K20/158A23K50/00
CPCA01K67/027A23K10/20A23K10/22A23K10/37A23K10/30A23K20/158A23K50/00A01K2207/25A01K2227/106A01K2267/0362Y02P60/87
Inventor 任娇艳徐真真刘炜
Owner SOUTH CHINA UNIV OF TECH
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