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Establishment method of vaccinium myrtillus callus regeneration system

A technology of callus and establishment method, which is applied in the field of plant propagation, can solve the problems of low propagation efficiency, delay of breeding process, and inability to realize large-scale propagation, and achieve the effect of easy material acquisition and simplified operation procedures

Active Publication Date: 2022-07-08
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many laboratories in northern Europe have screened some excellent strains from the wild, due to the low propagation

Method used

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  • Establishment method of vaccinium myrtillus callus regeneration system
  • Establishment method of vaccinium myrtillus callus regeneration system
  • Establishment method of vaccinium myrtillus callus regeneration system

Examples

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Embodiment 1

[0041] A method for establishing a European bilberry callus regeneration system, the specific steps are as follows:

[0042] (1) The young stems and leaves of bilberry seedlings that grow well on sterile medium are used as explant materials (because this material is an explant material grown in a sterile environment, it is not necessary to Sterilize and sterilize), cut the young shoots into small pieces of about 0.8 cm, remove the edge of the leaves and cut them into squares of about 0.5 cm, and inoculate them into the callus-inducing medium for cultivation. The leaves are laid flat on the surface of the medium, and the front Downwards, with the nodes of the stem section facing downwards, cultured in the light for 16 h at 28°C every day, and after 6-9 d, explants with callus were obtained (such as figure 1 shown);

[0043] The callus-inducing medium is: WPM + 3% sucrose + 1% agar + 0.5 mg / L IBA + 2.0 mg / L ZT, pH = 5.2 ± 0.02;

[0044] (2) The callus-grown explants were tran...

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Abstract

The invention discloses a method for establishing an European vaccinium callus regeneration system, which comprises the following steps of: inoculating tender branches of European vaccinium seedlings which grow well under a sterile culture condition as explant materials into a culture medium to obtain explants with calluses, and continuously culturing to generate adventitious buds; the formula of the culture medium comprises WPM, 3% of cane sugar, 1% of agar, 0.5 mg/L IBA and 2.0 mg/L ZT, and the pH is 5.2 +/-0.02; when the adventitious buds grow to 2-3 cm, the adventitious buds are moved to a rooting culture medium for rooting induction. The formula of a rooting culture medium is WPM, 2% of sucrose, 1% of agar, 0.3 mg/L of IAA, 1.0 mg/L of KT and 0.50 g/L of activated carbon, and the pH is 5.2 +/-0.02. According to the method disclosed by the invention, the obtaining rates of the callus and the adventitious buds of the vaccinium myrtillus are respectively 100% and 95% or above. According to the method, only 2-3 months are needed from explants to soil cultivation seedling obtaining, and technical support is provided for clone propagation of vaccinium myrtillus.

Description

technical field [0001] The invention relates to the technical field of plant reproduction, in particular to a method for establishing a callus regeneration system of bilberry. Background technique [0002] Bilberry (Latin name: Vaccinium myrtillus ), also known as billberry, is a member of the Rhododendron family ( Ericaceae ) bilberry ( Vaccinium ) plant, is a low shrub native to northern Europe, it contains more than 30 kinds of anthocyanins, is the bilberry variety that contains the most anthocyanins, and is also one of the richest natural sources of anthocyanins, with a content as high as 37.47% , has high economic value, medicinal value, etc., and is listed as a Class 1 herb by the American Herbal Products Association ［1,2］ , is the most expensive bilberry on the market today, and the only bilberry variety that can be used as medicine today, most commonly used to treat eye diseases ［3］ . Modern medical research shows that bilberry fruit has hypoglycemic, anti-inf...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008Y02P60/40Y02A40/22
Inventor 崔富强宋艳平
Owner ZHEJIANG FORESTRY UNIVERSITY
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