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Non-diagnostic evaluation method after mouse back psoriasis model construction

A psoriasis, mouse technology, applied in the field of psoriasis research, can solve the problem of short, usually about five days to reach the peak of the phenotype, and achieve the typical and intuitive appearance of the phenotype, easy to apply medicine, easy to apply evenly, The effect of large skin area

Pending Publication Date: 2022-07-05
CHINA THREE GORGES UNIV
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Problems solved by technology

However, due to the actual needs and conditional constraints in the research process, we only selected the phenotypic changes of the back skin of the mice for scoring. In addition, considering the short pathogenic cycle of the induced mice, the peak of the phenotype can usually be reached in about five days , is quite different from the application of PASI scores in clinical patients. Therefore, in order to make our research data more effective and reflect the pathological phenotype changes of mice more accurately, we aimed at the psoriasis on the back of experimental mice. Disease model, invent a new scoring method

Method used

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  • Non-diagnostic evaluation method after mouse back psoriasis model construction
  • Non-diagnostic evaluation method after mouse back psoriasis model construction

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Embodiment Construction

[0022] The embodiments of the present invention will be described in detail below with reference to the examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be regarded as limiting the scope of the present invention.

[0023] The evaluation method after the establishment of the mouse back psoriasis model for non-diagnostic purposes includes the following links:

[0024] S1. Determine the percentage of the skin lesion area of ​​the mouse in the area where the drug was applied, and score it with 0-2 points according to the percentage of the skin lesion area B, where 0%=0 point, 1%-30%=1 point, 31% ~100% = 2 points;

[0025] S2, determine the erythema score E, E is divided into 0-2 points according to the color of the erythema, of which no erythema is 0 points, light red 1 point, red and darker 2 points;

[0026] S3. Determine the scale score D, which is divided into 0-3 points accord...

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Abstract

The invention provides a non-diagnostic evaluation method for a mouse back psoriasis model after construction, which comprises the following steps: S1, determining the percentage of a mouse skin lesion area in a drug application area skin lesion area, and scoring by 0-2 scores according to a skin lesion area proportion B, 0% = 0 score, 1%-30% = 1 score, and 31%-100% = 2 score; s2, determining an erythema score E, wherein the E is divided into 0-2 scores according to the color depth of the erythema; s3, a scale score D is determined, scoring is carried out according to the scale covering skin damage area, the scale covering with no scale is equal to 0 score, the scale covering with 30% of skin damage is equal to 1 score, the scale covering with 60% of skin damage is equal to 2 score, and the scale covering with 90% or above of skin damage is equal to 3 score; s4, determining a plaque score I, performing scoring by 0-3 scores according to the size of the plaque, and if no is equal to 0 score, determining the average diameter of the plaque as lt; 2 mm is equal to 1 minute, the average diameter of the plaque is 2-4 mm and is equal to 2 minutes, and the average diameter of the plaque is gt; 4 mm is equal to 3 minutes; s5, the skin lesion severity S is determined, S = B + E + D + I, the mouse back skin psoriasis inflammation severity is more accurately described and quantitatively compared according to the S score, and intuitive and accurate statistical analysis is facilitated.

Description

technical field [0001] The invention relates to the field of psoriasis research, in particular to a method for evaluating a non-diagnostic mouse back psoriasis model after construction. Background technique [0002] Psoriasis is an immune-mediated chronic inflammatory skin disease. In the study of psoriasis, the construction of animal models of psoriasis mainly relies on local drug induction and application at the gene level. We chose to apply imiquimod to the back skin of mice. After induction, erythema and scales were produced; histologically, it resulted in excessive proliferation of keratinocytes, neovascularization, and immune cell infiltration, with similar Phenotypic and pathological features of human psoriasis. Clinically, the patient's current condition is evaluated by scoring the patient's skin lesion area and skin lesion severity, that is, the PASI score. The method is to divide the human body into four parts: head and neck, trunk, upper extremities and lower e...

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Application Information

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IPC IPC(8): G16H50/50A01K67/02
CPCG16H50/50A01K67/02
Inventor 韩珊珊程美琦胡悦王琼
Owner CHINA THREE GORGES UNIV
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