Auricularia auricula melanin immobilization method and application of auricularia auricula melanin in jelly preparation
A technology of melanin and fungus, which is applied in the field of food processing, can solve the problems of large load of immobilized fungus melanin and insoluble black fungus melanin, and achieve the effects of improving human immunity, novel and unique preparation method, and delicate and smooth taste
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Embodiment 1
[0024] Example 1: Preparation of grape juice fungus melanin jelly
[0025] Product formula: carrageenan 0.7%, konjac flour 0.2%, white sugar 8%, citric acid 0.2%, immobilized fungus melanin gel microspheres 5%, grape juice 30%, sodium alginate 0.4%, water 55.5%.
[0026] (1) Black fungus raw material pretreatment: the black fungus raw material is a dry product, pulverized by a pulverizer, and passed through a 60-mesh sieve to obtain black fungus powder, which is stored under dry conditions for subsequent use.
[0027] (2) The process of extracting black fungus melanin by ultrasonic-assisted method: take the black fungus powder that has passed through a 60-mesh sieve after pulverization and add 1 mol / L NaOH solution according to the material-liquid ratio of 1:20 (g:mL), and ultrasonically break the wall (power 300W). , 40min), centrifuge at 4000r / min for 20min to take the supernatant, adjust the pH to 2 with hydrochloric acid, acid precipitation in a boiling water bath for 10h,...
Embodiment 2
[0038] The difference between Example 2 and Example 1 is that the conditions of the immobilized fungus melanin and the mass fraction of the jelly product formula are different, and other conditions are the same.
[0039] Product formula: carrageenan 0.6%, konjac flour 0.15%, white sugar 10%, citric acid 0.15%, immobilized fungus melanin gel microspheres 4%, grape juice 20%, sodium alginate 0.3%, water 64.8%.
[0040] Use sodium alginate and carrageenan as carriers to immobilize fungus melanin: dissolve sodium alginate 1 and carrageenan 1g in 100ml of sterile deionized water by heating to make a carrier sol and cool it; Mix the fungus melanin alkaline solution with the carrier sol, stir evenly, and slowly drop the mixture into the 4% calcium chloride solution with a 5ml syringe (with a needle size of 0.6×25mm), solidify for 60min, and make a The microspheres are filtered, and the calcium chloride solution on the surface of the microspheres is washed with sterile deionized water...
Embodiment 3
[0042]The difference between Example 3 and Example 1 is that the conditions of the immobilized fungus melanin and the formula mass fraction of the jelly product are different, and other conditions are the same.
[0043] Product formula: carrageenan 0.6%, konjac flour 0.2%, white sugar 12%, citric acid 0.2%, immobilized fungus melanin gel microspheres 6%, apple juice 25%, sodium alginate 0.4%, water 55.6%.
[0044] Immobilization of fungus melanin with sodium alginate as carrier:
[0045] 1.5g of sodium alginate was heated and dissolved in 100ml of sterile deionized water to make a carrier sol, and cooled; in a ratio of 1:3 by volume, the fungus melanin alkaline solution and the carrier sol were mixed, and after stirring, use a 5ml syringe ( The needle size is 0.6×25mm), the mixture is slowly dropped into 4% calcium chloride solution to make microspheres with a diameter of 2-3mm, solidified for 60min, filtered, and the microspheres are washed with sterile deionized water to rem...
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