Multi-channel fluorescence detection system and detection method

Abstract

The invention discloses a multichannel fluorescence detection system which comprises a fixed seat and an objective lens, the fixed seat is provided with at least two fluorescence detection channels for performing fluorescence detection on a sample to be detected, and a reflector is arranged between the objective lens and the fixed seat; the reflecting mirror is used for reflecting the exciting light generated by the fluorescence detection channel directly facing the reflecting surface of the reflecting mirror, so that the reflected exciting light passes through the objective lens to detect the sample to be detected, and is used for reflecting the detection light generated by the sample to be detected into the fluorescence detection channel after passing through the objective lens; the fixed seat is provided with a fluorescence detection switching mechanism which is used for driving the reflecting mirror to rotate so as to enable the reflecting surface of the reflecting mirror to sequentially face different fluorescence detection channels according to a set sequence. The invention also discloses a multi-channel fluorescence detection method. According to the multi-channel fluorescence detection system and the detection method disclosed by the invention, multiple fluorescent dyes can be simultaneously detected on a sample, the detection efficiency can be improved, and wavelength interference between channels can be avoided.

Description

technical field [0001] The invention belongs to the technical field of fluorescence detection, in particular to a multi-channel fluorescence detection system and a detection method. Background technique [0002] Most of the existing fluorescence detection devices are single-channel fluorescence detection devices. For example, a Chinese patent application with publication number CN111830001A discloses a fluorescence detection device, comprising: an excitation optical path hollow cavity and a detection optical path hollow cavity, the detection optical path hollow cavity is vertically arranged at the sample placement position, and the excitation optical path The hollow cavity and the detection optical path hollow cavity are arranged at a preset angle; the excitation optical path hollow cavity includes a first optical filter and a second optical filter, and a light outlet of the excitation optical path hollow cavity is provided with The first lens; after the light emitted by th...

AI Technical Summary

Problems solved by technology

The single-channel fluorescence detection device can only detect one fluorescent dye for the sample at a time, which has the disadvantage of low detection efficiency

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