Reagent card

Abstract

The invention discloses a reagent card which comprises a reagent card body, and the reagent card body is internally provided with a flow channel for reaction liquid to flow and a reaction liquid flow driving mechanism for driving the reaction liquid to flow in the flow channel. According to the reagent card disclosed by the invention, the flow channel is arranged in the reagent card body, and the reaction liquid flow driving mechanism is arranged to drive the reaction liquid to flow in the flow channel, so that in the flowing process of the reaction liquid, the reagent card body is directly heated by an external heating device, and different areas of the reagent card body are kept at different set temperatures; therefore, the reaction liquid can be rapidly heated or cooled after flowing to the different areas so as to meet the detection requirement, the heating and cooling rate of the reaction liquid can be effectively improved, the reaction time can be shortened, and the reaction efficiency can be improved.

Description

technical field [0001] The invention belongs to the technical field of medical devices, in particular to a reagent card. Background technique [0002] The polymerase chain reaction is referred to as PCR (Polymerase Chain Reaction). PCR is a method of enzymatically synthesizing specific DNA fragments in vitro. Periodic cycle treatment is performed through steps such as heating at different temperatures, so that the target DNA can be rapidly amplified. PCR has the characteristics of strong specificity, high sensitivity, simple operation and time saving. It can not only be used for basic research such as gene isolation, cloning and nucleic acid sequence analysis, but also for disease diagnosis. [0003] The traditional PCR device heats the chip by air to realize the PCR reaction. On the one hand, due to the uneven heating of the air, the temperature in different regions cannot be reached quickly, making it difficult to ensure temperature control; on the other hand, the large ...

AI Technical Summary

Problems solved by technology

[0005] 1. The reaction cup containing the PCR reaction liquid is placed in a fixed position, and the temperature of the air is raised and lowered by the semiconductor heating plate. When the positive and negative electrodes of the semiconductor heating plate are reversed, the PCR reaction liquid is cooled. When the negative electrode is positively connected, heat the PCR reaction solution; the disadvantage is that it takes a certain amount of time for the temperature to rise and fall in the same space, and the longer the heating and cooling time, the longer the PCR reaction time;

[0006] 2. Set up two constant temperature zones, move the cuvette containing the PCR reaction solution to the two temperature zones at different times, and raise and lower the temperature of the PCR reaction solution through the two temperature zones; the disadvantage is: the cuvette The movement of the cuvette requires an external complex drive device. Although the method of moving the cuvette will heat up and down faster than the first way to a certain extent, this kind of temperature rise and fall is still achieved through the heat transfer of the air. The air temperature The heat is transferred to the reaction cup, and then the reaction cup is transferred to the PCR reaction solution. The heat transfer process is complicated and its heat transfer efficiency is affected.

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