Method for creating and identifying cold-resistant germplasm of macadamia nuts
An identification method and nut technology, applied in the fields of botanical equipment and methods, planting beds, seed immunity, etc., can solve the problems of low cold resistance, and achieve the effect of improving the survival rate and reasonable soil structure.
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Embodiment 1
[0030] A method for creating and identifying cold-resistant germplasm of macadamia nuts, comprising the following steps:
[0031] (1) Seed selection: Select mature shelled macadamia nuts that are free of diseases and insect pests, and the surface of the shell is smooth and unbroken, and picked in the same year as seeds; the varieties of mature shelled macadamia nuts are H2 and HAES 900;
[0032] (2) Seed soaking treatment: soak the seeds in a propylene glycol solution with a propyl paraben concentration of 10 g / kg for 4 hours, take them out to dry, and obtain the surface-treated seeds; the volume ratio of the seeds to the propyl paraben solution is 1:5;
[0033] (3) Variable temperature treatment:
[0034]3.1) Cyclic heating and cooling: adjust the temperature of the periodic heating and cooling device to 8°C, and put the surface-treated seeds into the periodic heating and cooling device for 3 cycles of heating and cooling to obtain temperature-change treated seeds; cycle Th...
Embodiment 2
[0039] A method for creating and identifying cold-resistant germplasm of macadamia nuts, comprising the following steps:
[0040] (1) Seed selection: Select mature shelled macadamia nuts that are free of diseases and insect pests, and the surface of the shell is smooth and unbroken, and picked in the same year as seeds; the varieties of mature shelled macadamia nuts are H2 and HAES 900;
[0041] (2) Seed soaking treatment: soak the seeds in a propylene glycol solution with a propyl paraben concentration of 20 g / kg for 6 hours, take them out to dry, and obtain the surface-treated seeds; the volume ratio of the seeds to the propyl paraben solution is 1:5;
[0042] (3) Variable temperature treatment:
[0043] 3.1) Cyclic heating and cooling: adjust the temperature of the periodic heating and cooling device to 12°C, and put the surface-treated seeds into the periodic heating and cooling device for 5 cycles of heating and cooling to obtain temperature-change treated seeds; cycle ...
Embodiment 3
[0048] A method for creating and identifying cold-resistant germplasm of macadamia nuts, comprising the following steps:
[0049] (1) Seed selection: Select mature shelled macadamia nuts that are free of diseases and insect pests, and the surface of the shell is smooth and unbroken, and picked in the same year as seeds; the varieties of mature shelled macadamia nuts are H2 and HAES 900;
[0050] (2) Seed soaking treatment: soak the seeds in a propylene glycol solution with a propyl paraben concentration of 15 g / kg for 5 hours, take them out and dry them to obtain surface-treated seeds; the volume ratio of the seeds to the propyl paraben solution is 1:5;
[0051] (3) Variable temperature treatment:
[0052] 3.1) Cyclic heating and cooling: adjust the temperature of the periodic heating and cooling device to 10°C, and put the surface-treated seeds into the periodic heating and cooling device for 4 cycles of heating and cooling to obtain temperature-change treated seeds; cycle ...
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