Compound microbial agent for degrading petroleum hydrocarbon as well as preparation method and application of compound microbial agent

Abstract

The invention discloses a compound microorganism bacterium agent for degrading petroleum hydrocarbon as well as a preparation method and application of the compound microorganism bacterium agent. The compound microorganism bacterium agent is prepared from active acinetobacter KJ-1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.20664, Providencia reevei L1 with the preservation number of CGMCC No.16526, bacillus SWH-1 with the preservation number of GMCC No.1950 and sphingobacterium SWH-2 with the preservation number of CGMCC No.1951. The petroleum hydrocarbon degradation composite microbial agent prepared by the invention can effectively degrade petroleum hydrocarbon substances in soil and oily sludge sand, can be effectively applied to biodegradation treatment of petroleum-polluted soil, and does not cause secondary pollution to the ecological environment of the soil; the synergistic effect of the acinetobacter live KJ-1, the province reevesii L1, the bacillus SWH-1 and the sphingobacterium SWH-2 is fully utilized, the final degradation efficiency of the petroleum hydrocarbon is improved, the preparation process of the solid microbial inoculum is simple and easy to operate, the cost is low, and the solid microbial inoculum is suitable for industrial production. The method has a wide application prospect in the field of bioremediation of petroleum-contaminated soil and petroleum product leakage accident sites.

Description

technical field [0001] The invention belongs to the technical field of microbes and soil remediation, and in particular relates to a petroleum hydrocarbon degrading composite microbial bacterial agent and its preparation method and application. Background technique [0002] Oil pollution has become a global environmental problem. According to statistics, the area of ​​oil-contaminated soil in my country's oil fields exceeds 300 million m 2 Every year, more than 1 million tons of oil-contaminated soil and more than 10 million tons of various oily sludge have been added each year, forming very serious soil oil pollution. The quality of the ecological environment in major oilfield areas continues to decline, the accumulation of toxic and harmful petroleum pollutants continues, and the ecological risks are increasing day by day. Due to the complexity of the soil medium, remediation of oil-contaminated soil has become a bottleneck problem in the international oilfield environme...

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Problems solved by technology

Physical and chemical repair methods are difficult to avoid secondary pollution, so that the original habitat is destroyed, and it is difficult to be effective and applied to large-scale, medium-low concentration oil pollution control, and the cost is expensive