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Early warning method for paralytic saxitoxin in bivalve mollusks

A shellfish toxin, bivalve mollusk technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve problems such as the inability to realize easy-to-operate, economical, sensitive, accurate and efficient early monitoring and early warning, and achieve economical and economical toxin extraction. , rapid detection, easy operation effect

Pending Publication Date: 2022-05-27
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an early warning method for paralytic shellfish toxins in bivalve molluscs, so as to solve the problems existing in the prior art that cannot realize convenient, economical, sensitive, accurate and efficient early monitoring and early warning. question

Method used

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  • Early warning method for paralytic saxitoxin in bivalve mollusks
  • Early warning method for paralytic saxitoxin in bivalve mollusks

Examples

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Embodiment 1

[0017] Example 1: Establish a method

[0018] Early warning methods for paralytic shellfish toxins in bivalve shellfish include the following steps:

[0019] 1) Feeding 40 bivalve shellfish under laboratory detoxification conditions for one week;

[0020] 2) The poisonous algae producing paralytic shellfish toxin were fed continuously for 5 days at the same concentration every day, and 10 were sampled at 4 different time periods on the 0th day, 1 day, 3 days and 5 days. Store the digestive glands separate from the remaining tissue at -20 °C when sampling;

[0021] 3) Add 0.1% of the formic acid solution homogenization to the tissues obtained in step 2) according to the ratio of 1:2 (tissue weight / g: reagent / mL), after 24 h of maceration at 4 °C, centrifuge at 12000 r / min for 10 min and aspirate the supernatant.

[0022] 4) Add the supernatant obtained in step 3) to the pre-activated well Purification is performed in the HLB solid phase extraction column.

[0023] 5) The purified li...

Embodiment 2

[0030] Example 2: Ezo scallops are detected as samples

[0031] Taking Ezo scallops as an example, the detection is carried out according to the method of Example 1.

[0032] 1. Feed 40 Ezo scallops for one week under laboratory detoxification conditions;

[0033] 2. Ezo scallops were continuously fed at a daily concentration of 3000 cell / mL with paralyzing shellfish toxin-producing algae for 5 days, and 10 scallops were sampled on the 0th, 1st, 3rd and 5th days of feeding, respectively. Store the digestive glands separate from the remaining tissue at -20 °C when sampling;

[0034] 3. Add 0.1% of the formic acid solution to the sample tissue obtained in step 2 according to the ratio of 1:2 (tissue weight / g: reagent / mL), homogenize with a tissue homogenizer, and extract the supernatant after 24h at 4 °C, centrifuge at 12000 revolutions for 10 min.

[0035]4. Add the supernatant obtained in step 3 to activate it with 6mL methanol and 6mL of water in advance Purification is performe...

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Abstract

The invention provides an early warning method for paralytic saxitoxin in bivalve mollusks. Whether the risk that the content of paralytic saxitoxin exceeds the standard exists or not is determined by detecting the content of paralytic saxitoxin in digestive glands of the bivalve mollusks. According to the method, the digestive gland is used as a monitoring sample, in toxin extraction operation, compared with integral soft tissue used in a conventional detection method, homogenate grinding is easier, operation is easy and convenient, and time and labor are saved. The volume of a detection sample is smaller, and toxin extraction is more economical. The accumulation of the digestive adenosine toxin is quicker and the accumulation amount is high, so that the toxin is more easily and sensitively detected. Meanwhile, compared with monitoring of the toxic algae level in a water body and a shellfish organism biomarker, the digestive gland is used as a toxin monitoring sample, interference of other factors is small, and the shellfish toxin level can be reflected more directly.

Description

Technical field [0001] The present invention belongs to the field of monitoring and early warning technology for shellfish toxin disasters, specifically relates to an early warning method of paralytic shellfish toxin in bivalve shellfish. Background [0002] Paralytic shellfish toxins are a class of marine biological toxins produced mainly by harmful poisonous algae that can be enriched into bivalve shellfish through the food chain, causing food safety problems. Paralytic shellfish toxins have become mandatory in domestic and international shellfish trade. [0003] Conventional paralytic shellfish toxin detection methods use the overall soft tissue of bivalve shellfish as sample tissue. However, the applicant's results show that there are significant differences in the accumulation of paralytic shellfish toxins in different tissues of bivalve shellfish, especially the high proportion of tough tissues such as mantle membranes in the entire soft tissue but the amount of toxin accum...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/72G01N2030/027G01N2030/062G01N2030/146Y02A40/81
Inventor 胡晓丽史姣霞孟德婷李茉莉王慧贞包振民
Owner OCEAN UNIV OF CHINA
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