Hair washing device for solid-phase microporous reactor and use method

Abstract

The invention discloses a head washing device for a solid-phase microporous reactor and a use method, the head washing device comprises a hollow solid-phase micropore with a top opening, and a gain structure arranged on the bottom surface of a micropore cavity; the hair washing liquid injection structure goes deep into the micropore cavity and keeps a certain distance from the inner side wall of the micropore cavity to form a washing liquid cavity; the waste liquid suction and discharge structure comprises a waste liquid guide-out cavity located in a washing area formed by the micropore cavity and the washing liquid cavity, the side wall, stretching into the micropore cavity, of the waste liquid guide-out cavity is axially connected with an extension edge, and the shape of the extension edge is matched with the shape of the gain structure; the hair washing and liquid injection structure and the waste liquid suction and discharge structure are combined together and then moved into the solid phase micropores, washing and suction actions can be synchronously carried out or rapidly and alternately carried out, the washing and suction actions can be rapidly completed in a short time, and compared with an existing washing mode that two steps of intermittent liquid suction and liquid adding are alternately carried out, the washing time is shortened, and the washing efficiency is improved. And the working efficiency and effect are improved.

Description

technical field [0001] The invention relates to a microporous hair washing device, in particular to a solid phase microporous reactor hair washing device and a use method. Background technique [0002] The enzyme-linked immunoassay technology based on solid-phase micropores and tubes has been applied in the fields of biological science research and clinical diagnosis for nearly half a century, and some emerging technology platforms are also realized based on solid-phase micropores and tubes. [0003] During the manufacture and detection of labeled immunoassay reagents for solid-phase micropores and tubes, the cleaning process of solid-phase micropores and tubes is a very important operation step. In this way, the free and unreacted components are fully separated and thoroughly washed to eliminate the free components that interfere with the detection and reduce the background to the lowest level to ensure the sensitivity of the detection method. The ideal washing effect is t...

AI Technical Summary

Problems solved by technology

The structure and devices of these two washing methods are relatively complicated, and take up more time, and there will be residual washing liquid.

[0005] These liquid injection needles and liquid withdrawal needles are all straight barrel structures with thin circumferences. The inner diameter of the liquid withdrawal needles is much smaller than the inner diameter of the micropores, and the suction force on the reaction interface attachments on the surrounding walls is weak; the order of liquid extraction is the contact surface type from top to bottom. However, the lower stratified suction has weak suction on the bottom of the micropore and the reaction interface on the side wall, and the washing is not sufficient; filling, standing, and pumping are the basic operating procedures and requirements, which consume a lot of time; The use of two-step suction and discharge of waste liquid in the micropores prolongs the washing time and reduces the washing efficiency; and the amount of liquid added should be controlled, and suction should be ensured to minimize residue; there is no special effect on the reaction interface between filling and pumping. Taking into account, it is also a time-consuming, inefficient and prone to failure link in traditional solid-phase microwells, tube reagent production, automated production of detection, and detection and analysis instruments

The defects of these traditional automatic washing devices have restricted and hindered the value of enzyme-linked immunoassay technology products based on solid-phase micropores and tubes to a certain extent.

Images