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Method for detecting lung cancer gene fusion based on NanoString platform

A gene fusion and detection method technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of low sensitivity

Pending Publication Date: 2022-04-29
HENAN CANCER HOSPITAL
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  • Description
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  • Application Information

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Problems solved by technology

NanoString can judge the occurrence of fusion by detecting whether the expression of the 5' and 3' ends of the gene is unbalanced, so as to detect the unknown fusion type. Small differences in background expression lead to low sensitivity of the method for detection of unknown ROS1 fusions

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  • Method for detecting lung cancer gene fusion based on NanoString platform
  • Method for detecting lung cancer gene fusion based on NanoString platform
  • Method for detecting lung cancer gene fusion based on NanoString platform

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Embodiment Construction

[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0028] The NanoString platform of the present invention is used to detect the probe detection method of lung cancer gene fusion, and the concrete steps of described method are as follows:

[0029] 1. Collection of gene fusion information and determination of housekeeping genes

[0030] Based on the fusion recommended by the NCCN Guidelines for the Diagnosis and Treatment of Non-Small Cell Lung Cancer (2020.v2), the fusion information of ALK, RET, MET, ROS1, and NTRK1 / 2 / 3 was det...

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Abstract

The invention discloses a detection method for detecting lung cancer gene fusion based on a NanoString platform. The method comprises the following steps: 1, collecting gene fusion information and determining a housekeeping gene; 2, designing a probe; 3, establishing a positive threshold calculation model and judging a positive result; most fusion types related to current lung cancer targeted therapy are collected, upstream and downstream sequences at each gene fusion breakpoint are determined, and a probe is designed based on an elementary method. The fusion with other lung cancer related to detection based on a NanoString platform is more comprehensive, especially in the fusion aspect of the four genes of MET and NTRK1 / 2 / 3; the mode of detecting whether fusion occurs or not by detecting whether the 5'end and the 3 'end of the ROS1 gene are unbalanced or not is a problem all the time, the detection sensitivity is very low, a threshold value is very difficult to judge, the algorithm is optimized again, and a new positive judgment standard is determined.

Description

technical field [0001] The invention relates to the field of lung cancer detection, in particular to a method for detecting lung cancer gene fusion based on the NanoString platform, which is used to detect lung cancer-related gene fusion to guide targeted therapy. Background technique [0002] Targeted therapy of lung cancer is an important form of lung cancer treatment. Targeted therapy is a treatment modality that targets well-identified oncogenic driver genes. The mutation types of oncogenic driver genes mainly include mutation, fusion and amplification. The proportion of fusion-driven mutations in lung cancer reaches 6%-10%, and related targeted drugs have been approved for marketing, so lung cancer patients can benefit from it. [0003] At present, a variety of molecular detection techniques are used in the detection of gene fusions in lung cancer, mainly including immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR) a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11G16B25/20G16B40/00
CPCC12Q1/6886G16B25/20G16B40/00C12Q2600/166
Inventor 孙瑞王志中马杰赵九洲郭永军冯君楠
Owner HENAN CANCER HOSPITAL
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