Microscope rapid and accurate automatic focusing method and system, computer equipment and medium

Abstract

The invention belongs to the technical field of image analysis, and discloses a rapid and accurate automatic focusing method and system for a microscope, computer equipment and a medium, and the method comprises the steps: continuously changing the distance between a photographed object and a lens of the microscope through employing a focusing depth method, searching a nearest clear image position, and carrying out the collection of an image; cutting the collected images, obtaining a quality evaluation value of each image through an image evaluation function, determining an image with the best quality according to the quality evaluation value of each image, and obtaining a focal length f0 corresponding to the image with the best quality; adopting a classification defocus depth method to judge whether the image belongs to a focusing category; and switching to the next visual field, and judging by using the classification defocus depth method again until the global scanning is finished. According to the invention, the precision and the efficiency can be balanced, and the focusing depth method and the classification defocusing depth method are combined to realize the accurate acquisition of the detection target, so that the detection target information and the pre-judgment scheme can be accurately obtained, the labor cost is reduced, and the high automation of the microscope is realized.

Description

Technical field [0001] The present invention belongs to the field of image analysis technology, in particular to a microscope fast and accurate autofocus method, system, computer equipment and media. Background [0002] At present, microscopic optical imaging technology is a comprehensive discipline integrating optics, machinery, electronics, sensors and computer technology. It plays an important role in the fields of integrated circuits, biomedicine, materials and molecules. In microscopic optical imaging digitization systems, the most critical focus strategy requires a balance of accuracy and speed. The traditional artificial microscope operation method has the disadvantages of low efficiency, low accuracy, long time, and inability to re-see. The fully automatic microscopic optical imaging digitization system can realize functions such as automatic focus, automatic global scanning, and intelligent analysis. Assistance with medically assisted diagnosis. [0003] In the field of...

AI Technical Summary

Problems solved by technology

[0005] Through the above analysis, the problems and defects of the existing technology are: the existing focusing method has low precision and low efficiency, and cannot perform microscope focusing efficiently and accurately

[0006] The difficulty of solving the above problems and defects is: unable to focus quickly and accurately

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