Octadecapeptide for improving cognitive impairment as well as preparation method and application of octadecapeptide

A technology for improving cognition and dysfunction, applied in the field of protein, can solve problems such as no cognitive dysfunction in the elderly, and achieve the effects of improving cognitive dysfunction, inhibiting apoptosis, and intervening in cell aging

Pending Publication Date: 2022-04-22
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no research report on oyster and its related products improving cognitive dysfunction in the elderly

Method used

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  • Octadecapeptide for improving cognitive impairment as well as preparation method and application of octadecapeptide
  • Octadecapeptide for improving cognitive impairment as well as preparation method and application of octadecapeptide
  • Octadecapeptide for improving cognitive impairment as well as preparation method and application of octadecapeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Preparation of Oyster Active Peptides for Improving Cognitive Impairment

[0069] 1. Preparation of oyster protein enzymatic hydrolysate (OPEH): after natural thawing of oysters, add water at a ratio of 1:3 (w / v), then use a high-speed homogenizer to homogenize on an ice bath for 5 minutes, and use 0.1mol / L NaOH to adjust the pH to 12.0. After stirring at 4°C for 3 hours, centrifuge at 10,000r / min at 4°C for 20min. Take the supernatant and adjust the pH to 4.8 with 0.1mol / L HCL, and then centrifuge at 10,000r / min at 4°C for 20min. , take the precipitate and add water (1:3 (w / v)), adjust the pH to 7.0, add neutral protease according to the ratio of enzyme to substrate (E / S) 2:100, enzymatically hydrolyze at 50°C for 4h, then inactivate the enzyme at 100°C for 10min , The supernatant obtained by centrifugation (10000r / min, 20min) is the oyster protein hydrolyzate.

[0070] Preparation of the ultrafiltration fraction of OPEH: Under ice bath conditions, the OPEH...

Embodiment 2

[0109] The influence of embodiment 2 synthetic peptides on BV2 cell viability

[0110] Determination of five different concentrations (1.0,0.5,0.1,0.05,0.01μg / mL) of 18 peptides on the BV2 cell viability (method is the same as in Example 1 step 3), the results are as follows Figure 17 Shown: After adding 18 peptides at five different concentrations of 1.0, 0.5, 0.1, 0.05, and 0.01 μg / mL, there was no significant difference in the viability of BV2 cells compared with the CT group, and there was no toxic side effect on BV2 cells.

Embodiment 3

[0111] Example 3 Effect of Synthetic Peptides on LPS-Induced BV2 Cells Secreting NO

[0112] Determination of five different concentrations (1.0,0.5,0.1,0.05,0.01μg / mL) of 18 peptides on the influence of NO content in BV2 cells induced by LPS (method is the same as in Example 1 step 3), the results are as follows Figure 18 As shown: five concentrations of 18 peptides (1.0, 0.5, 0.1, 0.05, 0.01 μg / mL) can significantly reduce the NO content produced by LPS-stimulated BV2 cells (P<0.001), and have a significant inhibitory effect on LPS-stimulated BV2 cells. NO production.

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Abstract

The invention belongs to the technical field of protein, and discloses oyster octadecapeptide for improving cognitive impairment as well as a preparation method and application of the oyster octadecapeptide. According to the octadecapeptide, by inhibiting generation of NO and ROS in cells, phosphorylation of NF-kappa B P65 and degradation of I kappa B are inhibited, and oxidative stress and chronic growth inflammation in the cells are reduced; by activating an ERK-CREB signal channel and inhibiting an apoptosis channel, proliferation and differentiation of nerve cells are promoted, the synaptic function is improved, cell senescence is intervened, and apoptosis is inhibited, so that the nerve cell protection effect is achieved, and cognitive impairment induced by senescence is improved.

Description

technical field [0001] The invention belongs to the technical field of proteins, and in particular relates to an octadecapeptide for improving cognitive dysfunction and its preparation method and application. Background technique [0002] Human aging is a complex natural phenomenon, often accompanied by a series of health problems, and cognitive function decline is one of its notable features. Aging of the brain is associated with behavioral deficits, memory loss and cognitive dysfunction. Cognitive impairment can be seen in older patients with mild cognitive impairment (MCI), as well as in patients with early Alzheimer's disease (AD). Among the elderly population over 65 years old, 15% to 20% suffer from mild cognitive impairment, and 32% of mild cognitive impairment patients will develop Alzheimer's disease within 5 years. With the increasingly serious population aging in recent years, the number of patients suffering from senile cognitive dysfunction is increasing. It h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P25/28A61P29/00C12P21/06C07K1/34C07K1/36C07K1/18C07K1/16A23L33/18
CPCC07K7/08A61P25/28A61P29/00C12P21/06A23L33/18A61K38/00A23V2002/00A23V2200/322A23V2250/55
Inventor 朱国萍章超桦卢虹玉曹文红秦小明郑惠娜高加龙林海生曾少葵
Owner GUANGDONG OCEAN UNIVERSITY
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