Method for constructing animal model with glaucoma-ciliary cyclitis syndrome and animal model

A technology of cyclitis and animal model, applied in animal husbandry and other directions, can solve the problem of detecting HSV in patient's aqueous humor

Active Publication Date: 2022-04-19
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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Problems solved by technology

However, follow-up studies failed to detect HSV in the patient's aqueous humor

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  • Method for constructing animal model with glaucoma-ciliary cyclitis syndrome and animal model
  • Method for constructing animal model with glaucoma-ciliary cyclitis syndrome and animal model
  • Method for constructing animal model with glaucoma-ciliary cyclitis syndrome and animal model

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[0043] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. The described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0044] A method for constructing an animal model of glaucoma-cyclitis syndrome of the present invention comprises the following steps:

[0045] The mice were anesthetized by intraperitoneal injection of 1% pentobarbital sodium (50 mg / kg), the eyeballs of the mice were kept slightly convex, and the mice were fixed on a brain stereotaxic apparatus. The right eye of the mouse was topically anesthetized with 0.5% proparacaine hydrochloride eye drops. Inhale 1 μL of MCMV gene recombinan...

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Abstract

The invention discloses a construction method of a glaucoma-ciliary cyclitis syndrome animal model and the animal model, and the construction method comprises the following steps: 1) animal anesthesia: anesthetizing an animal, and keeping eyeballs of the animal to be slightly convex; 2) eye anesthesia: performing surface anesthesia on the left eye and/or the right eye of the animal as a tested eye; and 3) virus infection: injecting the MCMV gene recombinant virus expressing the EGFP into the anterior chamber through the cornea center of an animal test eye, and establishing an animal model infected by the MCMV. According to the construction method of the animal model of the glaucoma-ciliary cyclitis syndrome, the mouse cytomegalovirus is injected into the anterior chamber of the eye of the mouse, the PSS animal model is constructed, and an effective animal model is provided for exploration of pathogenesis of the PSS.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and specifically refers to a method for constructing an animal model of glaucoma-cyclitis syndrome and the animal model. Background technique [0002] In the field of medicine, in order to facilitate the research of a certain disease, people have created a variety of animal models of diseases, such as animal models of cytomegalovirus (CMV) retinitis, animal models of heart failure, animal models of diabetes and so on. For diseases caused by viruses, if the virus is injected into the diseased site and can cause clinically similar symptoms and signs, it can be considered as a successful modeling. For example, in the mouse model of CMV retinitis, the virus is injected between the retina and the choroid through the supraciliary space to induce retinitis. It has not been found yet, but there are studies on animal models of glaucoma-cyclitis syndrome. [0003] Glaucoma cyclocytic sy...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/0275A01K2207/30A01K2217/20A01K2227/105A01K2267/03
Inventor 刘海霞刘昕王华东孙兴怀徐富强马琳应敏
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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