Fluorescent vector for marking powdery mildew of rubber tree as well as expression method and application of fluorescent vector

A technology of rubber tree powdery mildew and powdery mildew, applied in the direction of microorganism-based methods, fluorescence/phosphorescence, biochemical equipment and methods, etc., can solve the problem that foreign genes cannot be expressed, transformed genes cannot be stably inherited, and cannot be well guaranteed Transformation success rate and other issues, to achieve the effect of high promoter activity

Pending Publication Date: 2022-04-12
HAINAN UNIVERSITY
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Problems solved by technology

[0004] At present, the transgenic technology of powdery mildew still has some shortcomings and limitations, such as unstable expression of vectors, stable inheritance of transfo...

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  • Fluorescent vector for marking powdery mildew of rubber tree as well as expression method and application of fluorescent vector
  • Fluorescent vector for marking powdery mildew of rubber tree as well as expression method and application of fluorescent vector
  • Fluorescent vector for marking powdery mildew of rubber tree as well as expression method and application of fluorescent vector

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[0023] The technical solutions of the present invention will be further described below with reference to the accompanying drawings, but it should be covered by modifying or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the present invention, without departing from the spirit and scope of the present invention. In the scope of protection.

[0024] The present invention provides a fluorescent carrier labeled rubber tree white powder, which facilitates controlling conversion quality in the process of rubber tree white powder, calculating the transformation efficiency of white powder. The fluorescent carrier is a plasmid pJNARG-RP60-GFP suitable for use in fluorescent labeling rubber stabilica based on PJNARG plasmid. The plasmid pJNARG-RP60-GFP was inserted into a multidamic prototype gene from the XCMI enzyme sorting point, that is, Tub2 bacteria spirit resistant genes from melons. The promoter of the germac...

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Abstract

The invention discloses a fluorescent vector for marking powdery mildew of a rubber tree, the fluorescent vector is a plasmid pJNARG-RP60-GFP modified based on a pJNARG plasmid, a carbendazim resistance gene is inserted into the plasmid at an XcmI restriction enzyme cutting site, and a multiple cloning site of the plasmid pJNARG-RP60-GFP is between a promoter RP60 and a green fluorescent protein coding gene of the GFP. The invention provides a powerful tool for evaluating the conversion efficiency of the powdery mildew, i.e., plasmids containing fluorescent genes are converted by an electric shock conversion method, so that the plasmids express the fluorescent genes in powdery mildew cells, and the conversion efficiency of the powdery mildew is calculated by fluorescently labeling the converted powdery mildew cells. Therefore, the research on the effect of other exogenous or self genes simultaneously introduced into powdery mildew cells is facilitated.

Description

Technical field [0001] The present invention belongs to the technical field of transgenic pulse cells, which relates to a fluorescent carrier labeled rubber tree white powder. Background technique [0002] Polyfung fungi is a pathogenic fungi of plant white powder disease, which can cause serious production losses on important food crops and custries. The functional study of the petrococcus pathogenic gene is of great significance by transgenic technology. Because the whitefastic bacteria is a special living body parasitic, it is impossible to leave the plant living tissue culture, so it is difficult to perform transgenic operation. This is different from the amongweight parasitic fungi, and the incompatible parasitic fungi can be cultured in artificial medium, more conducive to genetic operation. [0003] The transgenic technology of white powder has an electric shock conversion method and a petrid-mediated transformation method. During gene conversion operation, it is necessary...

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Application Information

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IPC IPC(8): C12N15/80C12N15/65C12N1/15G01N21/64C12R1/645
Inventor 李潇殷金瑶董林朋刘玉涵刘文波缪卫国
Owner HAINAN UNIVERSITY
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