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Sample pad sealing agent as well as preparation method and application thereof

A sample pad and sealing agent technology, applied in the field of medical detection, can solve problems such as interference, achieve the effects of good stability, improve detection specificity and accuracy, and improve anti-interference ability

Pending Publication Date: 2022-03-29
NANJING VAZYME MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. There are false positives caused by the interference of antigen-antibody reactions: It has been reported that heterophilic antibodies or rheumatoid factors will interfere with commercial reagents used for detection

Method used

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  • Sample pad sealing agent as well as preparation method and application thereof
  • Sample pad sealing agent as well as preparation method and application thereof
  • Sample pad sealing agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Comparison of different concentrations of 2-ME in blocking agent without adding cleaning antibody composition

[0051] (1) Prepare 100mL 0.5mol / L Tris-HCl buffer solution: weigh 6.057g Tris, dissolve it completely with 70mL purified water, then adjust the pH to 7.48-7.52 with 5mol / L HCl solution, mix well, and use purified Dilute water to 100mL and let stand for use;

[0052] (2) Take a clean beaker, add purified water 60mL, 0.5mol / L Tris-HCl buffer 32mL, trehalose 16g, Tween-200.64mL, Procilin3000.64mL, add purified water to 160mL, and divide into 4 Add 16mg of RBC mab-clon7H3 to it, and then add 0mL, 0.16mL, 0.4mL, 0.8mL of 2-ME to it, mix thoroughly, add purified water to 80mL respectively, and the 4 parts of liquid are referred to as 0% (v / v) 2-ME, 0.2% (v / v) 2-ME, 0.5% (v / v) 2-ME, 1% (v / v) 2-ME;

[0053] (3) Take 45 mL of each of the 4 parts of liquid in step (2) and infiltrate 4 parts of sample pads respectively, and let stand for 5 minutes;

[0054]...

Embodiment 2

[0085] Example 2: Comparison of Cleaning Antibody Combinations

[0086] (1) Prepare 100mL 0.5mol / L Tris-HCl buffer solution: weigh 6.057g Tris, dissolve it completely with 70mL purified water, then adjust the pH to 7.48-7.52 with 5mol / L HCl solution, mix well, and use purified Dilute water to 100mL and let stand for use;

[0087](2) Add 100mL of purified water to the beaker, add 56mL of Tris-HCl buffer solution in step (1), 28g of trehalose, Tween-201.12mL, Procilin3001.12mL, add purified water to 280mL, and divide into 7 parts, add 0.4mL 2-ME, 16mg RBC mab-clon7H3 to each part, then add 16mg HBR-2, 16mg Tru Block-2, 16mg CAB mab-clon 24, 16mg HBR-2+16mg Tru Block- 2. 16mg HBR-2+16mg CAB mab-clon24, 16mg Tru Block 2+16mg CAB mab-clon 24, 16mg HBR-2+16mg Tru Block-2+16mgCAB mab-clon 24, mix well and add purified water To 80mL, the 7 liquids are referred to as H, T, 24, H+T, H+24, T+24, H+T+24 respectively;

[0088] (3) Take 45mL of each of the 7 parts of liquid in step (2) a...

Embodiment 3

[0109] Example 3: Evaluation of the interaction of reducing agents and cleaning antibodies

[0110] (1) Prepare 100mL 0.5mol / L Tris-HCl buffer solution: weigh 6.057g Tris, dissolve it completely with 70mL purified water, then adjust the pH to 7.48-7.52 with 5mol / L HCl solution, mix well, and use purified Dilute water to 100mL and let stand for use;

[0111] (2) Add 100 mL of purified water to the beaker, add 8 mL of Tris-HCl buffer solution in step (1), 4 g of trehalose, Tween-200.16 mL, Procilin 3000.16 mL, add purified water to 40 mL, and mix well Add 0.4mL 2-ME, 16mg RBC mab-clon7H3, then add 16mg HBR-2+16mg Tru Block-2+16mg CAB mab-clon24, mix well and add purified water to 80mL, referred to as 2-H+T+ twenty four;

[0112] (3) Take 45 mL of the liquid in step (2) and infiltrate 1 part of the sample pad, and let it stand for 5 minutes;

[0113] (4) Put the sample pad soaked in step (3) into an oven set at 37°C for 18 hours;

[0114] (5) Take out the sample pad dried in ...

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Abstract

The invention relates to a sample pad sealing agent as well as a preparation method and application thereof, and belongs to the field of medical detection. The invention aims to reduce the influence caused by interference factors in the detection process of immunochromatography and ensure the good stability of a sealed sample pad, and specifically, the sample pad sealing agent comprises a buffer solution, a protein protective agent, a surfactant, a preservative, a reducing agent, a coagulant and a cleaning antibody, wherein the cleaning antibody is selected from one or more of HBR (Hepatitis B Receptor)-2, Tru Block-2 and CAB (Cathode Activated Block) mab-Clon 24.

Description

technical field [0001] The invention belongs to the field of medical detection, and mainly relates to a sample pad sealant, a preparation method thereof and an immunochromatographic test strip or a test paper card using the sealant. Background technique [0002] Cardiac troponin I (cTnI) has been used as an important indicator for judging myocardial cell damage in acute myocardial infarction (AMI), myocarditis and other diseases, and is also recognized as assisting in acute coronary syndrome (acute coronary syndromes, ACS) risk stratification Therefore, it is very important to ensure the accuracy, specificity and stability of cTnI detection results in clinical application. [0003] One of the commonly used cTnI detection methods is immunochromatography. Immunochromatography (immunochromatography) technology is a fast, simple, sensitive, intuitive, low-cost detection method that can truly realize on-site detection. First fixed on a certain zone of the polymer membrane, when ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/53G01N33/68
CPCG01N33/558G01N33/5306G01N33/6893G01N33/6887G01N2800/324G01N2800/325G01N2800/52G01N2333/4712
Inventor 邵祥明唐波桑曼王霞琴
Owner NANJING VAZYME MEDICAL TECH CO LTD
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