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Bacillus subtilis as well as application and product thereof

A technology of Bacillus subtilis and products, which is applied in the field of microorganisms and can solve the problem of few species of Bacillus subtilis

Pending Publication Date: 2022-03-22
内蒙古科为博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few species of Bacillus subtilis that have been preserved in pure culture and have practical application value. For this field, there is no doubt that sufficient microbial resource reserves have important practical significance for its industrial application.

Method used

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  • Bacillus subtilis as well as application and product thereof
  • Bacillus subtilis as well as application and product thereof
  • Bacillus subtilis as well as application and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Example 1. Preservation and cultivation of Bacillus subtilis Pro-N2

[0099] 1. The deposit information of Bacillus subtilis Pro-N2 is:

[0100] Bacillus subtilis (Bacillus subtilis) Pro-N2 was deposited in the General Microbiology Center (CGMCC) of China Committee for Microorganism Culture Collection (CGMCC) on June 3, 2019, and its address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China Institute of Microbiology, Chinese Academy of Sciences, the deposit number is CGMCC No.17882.

[0101] 2. Fermentation of Bacillus subtilis Pro-N2:

[0102] (1) Strain activation: Bacillus subtilis Pro-N2 was inoculated on the slant medium, and cultured at 30-40°C for 12-24 hours;

[0103]The slant medium includes: beef extract 1.0-5.0g, soybean peptone 5.0-15.0g, yeast extract 1.0-5.0g, sodium chloride 1.0-5.0g, glucose 1.0-10.0g, agar 15-20g, distilled water 1000mL, pH is 7.0.

[0104] (2) Preparation of primary seed liquid: Inoculate the Bacillus subtilis ...

Embodiment 2

[0112] Example 2. Preservation and cultivation of Clostridium butyricum CC02001

[0113] 1. The deposit information of Clostridium butyricum CC02001 is:

[0114] Clostridium butyricum (Clostridium butyricum) CC02001 was deposited in the General Microbiology Center (CGMCC) of China Committee for Microorganism Culture Collection (CGMCC) on July 01, 2020. Its address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Chinese Academy of Sciences Institute of Microbiology, the deposit number is CGMCC No.20170.

[0115] 2. Fermentation of Clostridium butyricum CC02001:

[0116] (1) Strain activation: inoculate Clostridium butyricum CC02001 on the slant medium, and cultivate at 30-40°C for 12-24 hours;

[0117] The slant medium includes: beef extract 1.0-5.0g, soybean peptone 5.0-15.0g, yeast extract 1.0-5.0g, sodium chloride 1.0-5.0g, glucose 1.0-10.0g, agar 15-20g, distilled water 1000mL, pH is 7.0.

[0118] (2) First-level seed liquid preparation: inoculate the C...

Embodiment 3

[0126] Embodiment 3. the cultivation of Enterococcus faecalis CICC NO.24834

[0127] 1. Enterococcus faecalis CICC NO.24834 was purchased from China Industrial Microorganism Culture Collection Management Center.

[0128] 2. Fermentation of Enterococcus faecalis CICC NO.24834:

[0129] (1) Strain activation: inoculate Enterococcus faecalis on MRS solid slant medium, and culture it under aerobic or facultative conditions at 30-40°C for 12-24 hours;

[0130] The slant medium includes: peptone 10g, beef extract 5g, yeast extract 5g, glucose 20g, Tween-801mL, dipotassium hydrogen phosphate 2g, sodium acetate 5g, diammonium citrate 2g, magnesium sulfate 0.2g, manganese sulfate 0.05g, Calcium carbonate 6g, agar powder 16g, distilled water 1000mL, sterilized at 121°C for 30min.

[0131] (2) Preparation of seed solution: inoculate Enterococcus faecalis cultured on a slant into the seed medium, and cultivate under aerobic or facultative conditions at 30-40°C for 12-24 hours;

[0132]...

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Abstract

The invention provides bacillus subtilis Pro-N2 as well as application and a product thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus subtilis Pro-N2 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 17882. The bacillus subtilis Pro-N2 as well as the fermentation liquor and the product of the bacillus subtilis Pro-N2 have relatively strong intestinal tract colonization capability, can rapidly improve microbial balance in host intestinal tracts, can be applied to foods or food additives and feeds or feed additives, and are simple in preparation method and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus subtilis and its application and products. Background technique [0002] Bacillus subtilis is a species of Bacillus. Its single cell is 0.7-0.8×2-3 microns, and the coloring is uniform. Uncapsulated, with periosteum flagella, able to move. Gram-positive bacteria can form endogenous stress-resistant spores. The spores are 0.6-0.9×1.0-1.5 microns, oval to columnar, located in the center of the bacterium or slightly biased, and the bacterium does not expand after spore formation. Bacillus subtilis grows and reproduces quickly. The surface of the colony is rough and opaque, stained white or slightly yellow. When growing in a liquid medium, it often forms wrinkles. It is an aerobic bacterium. [0003] Bacillus subtilis can use protein, various sugars and starch to decompose tryptophan to form indole. Active substances such as subtilisin, polymyxin, ny...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10A23K10/16A23K10/18A23K20/163A23L29/00A23L29/30A23L33/135A23L33/21C12R1/125C12R1/01
CPCC12N1/20C12N11/10A23K10/18A23K10/16A23K20/163A23L33/135A23L33/21A23L29/065A23L29/30Y02P60/87
Inventor 吴培均奎奎罗建杰李富伟
Owner 内蒙古科为博生物科技有限公司
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